Effect of Copper Treatment on the Composition and Function of the Bacterial Community in the Sponge Haliclona cymaeformis
Tian, Ren-Mao1; Wang, Yong1,2; Bougouffa, Salim3; Gao, Zhao-Ming1,2; Cai, Lin1; Zhang, Wei-Peng1; Bajic, Vladimir3; Qian, Pei-Yuan1
2014-11-01
Source PublicationMBIO
Volume5Issue:6
AbstractMarine sponges are the most primitive metazoan and host symbiotic microorganisms. They are crucial components of the marine ecological system and play an essential role in pelagic processes. Copper pollution is currently a widespread problem and poses a threat to marine organisms. Here, we examined the effects of copper treatment on the composition of the sponge-associated bacterial community and the genetic features that facilitate the survival of enriched bacteria under copper stress. The 16S rRNA gene sequencing results showed that the sponge Haliclona cymaeformis harbored symbiotic sulfur-oxidizing Ectothiorhodospiraceae and photosynthetic Cyanobacteria as dominant species. However, these autotrophic bacteria decreased substantially after treatment with a high copper concentration, which enriched for a heterotrophic-bacterium-dominated community. Metagenomic comparison revealed a varied profile of functional genes and enriched functions, including bacterial motility and chemotaxis, extracellular polysaccharide and capsule synthesis, virulence-associated genes, and genes involved in cell signaling and regulation, suggesting short-period mechanisms of the enriched bacterial community for surviving copper stress in the microenvironment of the sponge. Microscopic observation and comparison revealed dynamic bacterial aggregation within the matrix and lysis of sponge cells. The bacteriophage community was also enriched, and the complete genome of a dominant phage was determined, implying that a lytic phage cycle was stimulated by the high copper concentration. This study demonstrated a copper-induced shift in the composition of functional genes of the sponge-associated bacterial community, revealing the selective effect of copper treatment on the functions of the bacterial community in the microenvironment of the sponge.
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1128/mBio.01980-14
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCORAL-REEF SPONGES ; MARINE SPONGES ; ESCHERICHIA-COLI ; SEQUENCES ; METAL ; MICROORGANISMS ; CONTAMINATION ; ACCUMULATION ; POPULATIONS ; ALIGNMENT
WOS SubjectMicrobiology
WOS IDWOS:000347073600023
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/771
Collection深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
Affiliation1.Hong Kong Univ Sci & Technol, Div Sci, Hong Kong, Hong Kong, Peoples R China
2.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, San Ya, Hai Nan, Peoples R China
3.KAUST, CBRC, Thuwal, Saudi Arabia
Recommended Citation
GB/T 7714
Tian, Ren-Mao,Wang, Yong,Bougouffa, Salim,et al. Effect of Copper Treatment on the Composition and Function of the Bacterial Community in the Sponge Haliclona cymaeformis[J]. MBIO,2014,5(6).
APA Tian, Ren-Mao.,Wang, Yong.,Bougouffa, Salim.,Gao, Zhao-Ming.,Cai, Lin.,...&Qian, Pei-Yuan.(2014).Effect of Copper Treatment on the Composition and Function of the Bacterial Community in the Sponge Haliclona cymaeformis.MBIO,5(6).
MLA Tian, Ren-Mao,et al."Effect of Copper Treatment on the Composition and Function of the Bacterial Community in the Sponge Haliclona cymaeformis".MBIO 5.6(2014).
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