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Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops
Zhao, Chao1,2; Deng, Yunjin1; Wang, Xingna3; Li, Qiuzhe1; Huang, Yifan1,2,4; Liu, Bin1,2
2014-09-01
发表期刊JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷号24期号:9页码:1280-1290
摘要In order to obtain the cellulolytic bacterial consortia, sediments from Great Basin hot springs (Nevada, USA) were sampled and enriched with cellulosic biomass as the sole carbon source. The bacterial composition of the resulting anaerobic ethanol-producing celluloytic bacterial consortium, named SV79, was analyzed. With methods of the full-length 16S rRNA library-based analysis and denaturing gradient gel electrophoresis, 21 bacteria belonging to eight genera were detected from this consortium. Clones with closest relation to the genera Acetivibrio, Clostridium, Cellulosilyticum, Ruminococcus, and Sporomusa were predominant. The cellulase activities and ethanol productions of consortium SV79 using different agricultural residues (sugarcane bagasse and spent mushroom substrate) and energy crops (Spartina anglica, Miscanthus floridulus, and Pennisetum sinese Roxb) were studied. During cultivation, consortium SV79 produced the maximum filter paper activity (FPase, 9.41 U/ml), carboxymethylcellulase activity (CMCase, 6.35 U/ml), and xylanase activity (4.28 U/ml) with sugarcane bagasse, spent mushroom substrate, and S. anglica, respectively. The ethanol production using M. floridulus as substrate was up to 2.63 mM ethanol/g using gas chromatography analysis. It has high potential to be a new candidate for producing ethanol with cellulosic biomass under anoxic conditions in natural environments.
文章类型Article
关键词Microbial Consortium Cellulolytic Bacterium Hot Springs Cellulase Ethanol
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.4014/jmb.1401.01022
收录类别SCI
语种英语
关键词[WOS]SP-NOV. ; LIGNOCELLULOSIC BIOMASS ; THERMOPHILIC MICROORGANISMS ; CLOSTRIDIUM-THERMOCELLUM ; BIOETHANOL PRODUCTION ; DEGRADING BACTERIA ; HUMAN FECES ; GEN.-NOV. ; PRETREATMENT ; BAGASSE
WOS类目Biotechnology & Applied Microbiology ; Microbiology
WOS记录号WOS:000342394900016
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.idsse.ac.cn/handle/183446/767
专题科学技术处
作者单位1.Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Fujian, Peoples R China
2.Natl Engn Res Ctr Juncao, Fuzhou 350002, Fujian, Peoples R China
3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China
4.Fujian Agr & Forestry Univ, Coll Anim Sci, Fuzhou 350002, Fujian, Peoples R China
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Zhao, Chao,Deng, Yunjin,Wang, Xingna,et al. Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2014,24(9):1280-1290.
APA Zhao, Chao,Deng, Yunjin,Wang, Xingna,Li, Qiuzhe,Huang, Yifan,&Liu, Bin.(2014).Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,24(9),1280-1290.
MLA Zhao, Chao,et al."Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 24.9(2014):1280-1290.
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