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Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops
Zhao, Chao1,2; Deng, Yunjin1; Wang, Xingna3; Li, Qiuzhe1; Huang, Yifan1,2,4; Liu, Bin1,2
2014-09-01
Source PublicationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume24Issue:9Pages:1280-1290
AbstractIn order to obtain the cellulolytic bacterial consortia, sediments from Great Basin hot springs (Nevada, USA) were sampled and enriched with cellulosic biomass as the sole carbon source. The bacterial composition of the resulting anaerobic ethanol-producing celluloytic bacterial consortium, named SV79, was analyzed. With methods of the full-length 16S rRNA library-based analysis and denaturing gradient gel electrophoresis, 21 bacteria belonging to eight genera were detected from this consortium. Clones with closest relation to the genera Acetivibrio, Clostridium, Cellulosilyticum, Ruminococcus, and Sporomusa were predominant. The cellulase activities and ethanol productions of consortium SV79 using different agricultural residues (sugarcane bagasse and spent mushroom substrate) and energy crops (Spartina anglica, Miscanthus floridulus, and Pennisetum sinese Roxb) were studied. During cultivation, consortium SV79 produced the maximum filter paper activity (FPase, 9.41 U/ml), carboxymethylcellulase activity (CMCase, 6.35 U/ml), and xylanase activity (4.28 U/ml) with sugarcane bagasse, spent mushroom substrate, and S. anglica, respectively. The ethanol production using M. floridulus as substrate was up to 2.63 mM ethanol/g using gas chromatography analysis. It has high potential to be a new candidate for producing ethanol with cellulosic biomass under anoxic conditions in natural environments.
SubtypeArticle
KeywordMicrobial Consortium Cellulolytic Bacterium Hot Springs Cellulase Ethanol
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.4014/jmb.1401.01022
Indexed BySCI
Language英语
WOS KeywordSP-NOV. ; LIGNOCELLULOSIC BIOMASS ; THERMOPHILIC MICROORGANISMS ; CLOSTRIDIUM-THERMOCELLUM ; BIOETHANOL PRODUCTION ; DEGRADING BACTERIA ; HUMAN FECES ; GEN.-NOV. ; PRETREATMENT ; BAGASSE
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000342394900016
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/767
Collection科学技术处
Affiliation1.Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Fujian, Peoples R China
2.Natl Engn Res Ctr Juncao, Fuzhou 350002, Fujian, Peoples R China
3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China
4.Fujian Agr & Forestry Univ, Coll Anim Sci, Fuzhou 350002, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Chao,Deng, Yunjin,Wang, Xingna,et al. Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2014,24(9):1280-1290.
APA Zhao, Chao,Deng, Yunjin,Wang, Xingna,Li, Qiuzhe,Huang, Yifan,&Liu, Bin.(2014).Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,24(9),1280-1290.
MLA Zhao, Chao,et al."Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 24.9(2014):1280-1290.
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