PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve
Liu, Helu1,2,3; Zhang, Hongkuan1; Zheng, Huaiping1,2; Wang, Shuqi1; Guo, Zhicheng1; Zhang, Guofan4; Zheng HP(郑怀平)
2014-12-24
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume62Issue:51Pages:12384-12391
Abstract

Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in important physiological processes. However, the endogenous PUFA biosynthesis pathway is poorly understood in marine bivalves. Previously, a fatty acyl desaturase (Fad) with ?5 activity was functionally characterized and an elongase termed Elovl2/5 was reported to efficiently elongate 18:2n-6 and 18:3n-3 to 20:2n-6 and 20:3n-3 respectively in Chlamys nobilis. In this study, another elongase and another Fad were identified. Functional characterization in recombinant yeast showed that the newly cloned elongase can elongate 20:4n-6 and 20:5n-3 to C22 and C24, while the newly cloned scallop Fad exhibited a ?8-desaturation activity, and could desaturate exogenously added PUFA 20:3n-3 and 20:2n-6 to 20:4n-3 and 20:3n-6 respectively, providing the first compelling evidence that noble scallop could de novo biosynthesize 20:5n-3 and 20:4n-6 from PUFA precursors though the "?8 pathway". No Delta 6 or Delta 4 activity was detected for this Fad. Searching against our scallop transcriptome database failed to find any other Fad-like genes, indicating that noble scallop might have limited ability to biosynthesize 22:6n-3. Interestingly, like previously characterized Elovl2/5, the two newly cloned genes showed less efficient activity toward n-3 PUFA substrates than their homologous n-6 substrates, resulting in a relatively low efficiency to biosynthesize n-3 PUFA, implying an adaption to marine environment.

SubtypeArticle
KeywordChlamys Nobilis Pufa Biosynthesis Delta 8 Pathway Marine Bivalve
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1021/jf504648f
Indexed BySCI
Language英语
WOS KeywordACYL DELTA-6 DESATURASE ; FATTY-ACID-COMPOSITION ; OCTOPUS-VULGARIS CUVIER ; FUNCTIONAL-CHARACTERIZATION ; TELEOST FISH ; MOLECULAR-CLONING ; FRESH-WATER ; ALGAL DIETS ; METABOLISM ; ELONGASE
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000347138500014
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/757
Collection深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
Corresponding AuthorZheng HP(郑怀平)
Affiliation1.Shantou Univ, Key Lab Marine Biotechnol Guangdong Prov, Shantou 515063, Peoples R China
2.Mariculture Res Ctr Subtrop Shellfish & Algae, Dept Educ Guangdong Prov, Shantou 515063, Peoples R China
3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
Liu, Helu,Zhang, Hongkuan,Zheng, Huaiping,et al. PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(51):12384-12391.
APA Liu, Helu.,Zhang, Hongkuan.,Zheng, Huaiping.,Wang, Shuqi.,Guo, Zhicheng.,...&郑怀平.(2014).PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(51),12384-12391.
MLA Liu, Helu,et al."PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.51(2014):12384-12391.
Files in This Item: Download All
File Name/Size DocType Version Access License
2-PUFA Biosynthesis (9985KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Helu]'s Articles
[Zhang, Hongkuan]'s Articles
[Zheng, Huaiping]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Helu]'s Articles
[Zhang, Hongkuan]'s Articles
[Zheng, Huaiping]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Helu]'s Articles
[Zhang, Hongkuan]'s Articles
[Zheng, Huaiping]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 2-PUFA Biosynthesis Pathway in Marine Scallop Chlamys nobilis Reeve.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.