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The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria
Tracey, John C.1; Coronado, Maricela1; Giessen, Tobias W.2,3; Lau, Maggie C. Y.1,5; Silver, Pamela A.4; Ward, Bess B.1
2019-12-27
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume9Pages:11
Abstract

Many prokaryotes encode protein-based encapsulin nanocompartments, including anaerobic ammonium oxidizing (anammox) bacteria. This study expands the list of known anammox encapsulin systems from freshwater species to include the marine genus Scalindua. Two novel systems, identified in "Candidatus Scalindua rubra" and "Candidatus Scalindua sp. SCAELEC01 167" possess different architectures than previously studied freshwater anammox encapsulins. Characterization of the S. rubra encapsulin confirms that it can self-assemble to form compartments when heterologously expressed in Escherichia coli. BLASTp and HMMER searches of additional genomes and metagenomes spanning a range of environments returned 26 additional novel encapsulins, including a freshwater anammox encapsulin identified in "Candidatus Brocadia caroliniensis". Phylogenetic analysis comparing these 28 new encapsulin sequences and cargo to that of their closest known relatives shows that encapsulins cluster by cargo protein type and therefore likely evolved together. Lastly, prokaryotic encapsulins may be more common and diverse than previously thought. Through searching a small sample size of all public metagenomes and genomes, many new encapsulin systems were unearthed by this study. This suggests that many additional encapsulins likely remain to be discovered.

DOI10.1038/s41598-019-56533-5
Indexed BySCI
Funding OrganizationGordon and Betty Moore Foundation
Language英语
Funding ProjectGordon and Betty Moore Foundation[5506]
WOS KeywordHYDROXYLAMINE OXIDOREDUCTASE ; STRUCTURAL BASIS ; EVOLUTION ; NITROSOMONAS ; KEY ; LOCALIZATION ; ENRICHMENT ; SEARCH ; ENZYME ; TOOLS
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000509340400001
PublisherNATURE PUBLISHING GROUP
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/7384
Collection深海科学研究部_地外海洋系统研究室
Corresponding AuthorTracey, John C.
Affiliation1.Princeton Univ, Dept Geosci, Guyot Hall, Princeton, NJ 08544 USA
2.Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
3.Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA
4.Wyss Inst Biol & Inspired Engn, 3 Blackfan Circle, Boston, MA 02115 USA
5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China
Recommended Citation
GB/T 7714
Tracey, John C.,Coronado, Maricela,Giessen, Tobias W.,et al. The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria[J]. SCIENTIFIC REPORTS,2019,9:11.
APA Tracey, John C.,Coronado, Maricela,Giessen, Tobias W.,Lau, Maggie C. Y.,Silver, Pamela A.,&Ward, Bess B..(2019).The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria.SCIENTIFIC REPORTS,9,11.
MLA Tracey, John C.,et al."The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria".SCIENTIFIC REPORTS 9(2019):11.
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