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A novel, kinetically stable copper, zinc superoxide dismutase from Psychropotes longicauda
Li, Yanan1,2; Zhang, Haibin1
2019-11-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume140Pages:998-1005
Abstract

Superoxide dismutases (SODs) are one of the most important antioxidant enzymes against oxidative damage. In the present study, we cloned and expressed a novel and stable Cu, Zn-SOD from a hadal sea cucumber Psychropotes longicauda (i.e., Pl-Cu, Zn-SOD). The purified recombinant enzyme was intracellular, dimeric with the Mr. of approximately 38 kDa, with the expressed activity from 0 degrees C to 60 degrees C at an optimal temperature of 20 degrees C and 30 degrees C and maximum activity at the pH of 8.0. The Km and Vmax values of Pl-Cu, Zn-SOD were 0.041 +/- 0.004 mM and 1450.275 +/- 36.621 U/mg, respectively. At tested conditions, Pl-Cu, Zn-SOD was relatively stable in chemicals, such as beta-ME, EDTA, Tween 20, Triton X-100, and Chaps, especially in urea and guanidine hydrochloride, which can resist protease hydrolysis and tolerate high hydrostatic pressure of 100 MPa and 2 M NaCl. All these properties make Pl-Cu, Zn-SOD a candidate in the biopharmaceutical and nutraceutical fields, and help us better understand the adaptation mechanism of hadal area. (C) 2019 Published by Elsevier B.V.

KeywordExpression Purification Deep-sea enzyme pCold vector The Mariana trench
DOI10.1016/j.ijbiomac.2019.08.089
Indexed BySCI
Funding OrganizationNational Key Research and Development Program of China ; Sanya Key Laboratory Program
Language英语
Funding ProjectNational Key Research and Development Program of China[2017YFC0306600] ; National Key Research and Development Program of China[2018YFC0309804] ; Sanya Key Laboratory Program
WOS KeywordCRYSTAL-STRUCTURE ; REACTIVE OXYGEN ; EXPRESSION ; CLONING ; STABILITY ; MECHANISM ; LIPASE ; INSIGHTS ; NEURONS ; STRESS
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000501656200106
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/7381
Collection研究生部
深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
Corresponding AuthorZhang, Haibin
Affiliation1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Corresponding Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
Li, Yanan,Zhang, Haibin. A novel, kinetically stable copper, zinc superoxide dismutase from Psychropotes longicauda[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,140:998-1005.
APA Li, Yanan,&Zhang, Haibin.(2019).A novel, kinetically stable copper, zinc superoxide dismutase from Psychropotes longicauda.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,140,998-1005.
MLA Li, Yanan,et al."A novel, kinetically stable copper, zinc superoxide dismutase from Psychropotes longicauda".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 140(2019):998-1005.
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