Vertical Profiles of Bacteria in the Tropical and Subarctic Oceans Revealed by Pyrosequencing
Jing, Hongmei1,2; Xia, Xiaomin1; Suzuki, Koji3; Liu, Hongbin1
2013-11-13
Source PublicationPLOS ONE
Volume8Issue:11
AbstractCommunity composition of Bacteria in the surface and deep water layers were examined at three oceanic sites in the Pacific Ocean separated by great distance, i.e., the South China Sea (SCS) in the western tropical Pacific, the Costa Rica Dome (CRD) in the eastern tropical Pacific and the western subarctic North Pacific (SNP), using high throughput DNA pyrosequencing of the 16S rRNA gene. Bioinformatic analysis rendered a total of 143600 high quality sequences with an average 11967 sequences per sample and mean read length of 449 bp. Phylogenetic analysis showed that Proteobacteria dominated in all shallow and deep waters, with Alphaproteobacteria and Gammaproteobacteria the two most abundant components, and SAR11 the most abundant group at family level in all regions. Cyanobacteria occurred mainly in the surface euphotic layer, and the majority of them in the tropical waters belonged to the GpIIa family including Prochlorococcus and Synechococcus, whilst those associated with Cryptophytes and diatoms were common in the subarctic waters. In general, species richness (Chao1) and diversity (Shannon index H') were higher for the bacterial communities in the intermediate water layers than for those in surface and deep waters. Both NMDS plot and UPGMA clustering demonstrated that bacterial community composition in the deep waters (500 m similar to 2000 m) of the three oceanic regions shared a high similarity and were distinct from those in the upper waters (5 m similar to 100 m). Our study indicates that bacterial community composition in the DOC-poor deep water in both tropical and subarctic regions were rather stable, contrasting to those in the surface water layers, which could be strongly affected by the fluctuations of environmental factors.
SubtypeArticle
WOS HeadingsScience & Technology
DOI10.1371/journal.pone.0079423
Indexed BySCI
Language英语
WOS KeywordNW MEDITERRANEAN SEA ; OXYGEN MINIMUM ZONE ; NORTH PACIFIC-OCEAN ; 16S RIBOSOMAL-RNA ; SOUTH CHINA SEA ; MICROBIAL DIVERSITY ; PHYLOGENETIC COMPOSITION ; COMMUNITY GENOMICS ; SPATIAL-PATTERNS ; SARGASSO SEA
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000327254700131
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/737
Collection深海科学研究部_深海生物学研究室_海洋微生物分子生态学研究组
Affiliation1.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China
2.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya, Peoples R China
3.Hokkaido Univ, Fac Environm Earth Sci, Sapporo, Hokkaido, Japan
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
Jing, Hongmei,Xia, Xiaomin,Suzuki, Koji,et al. Vertical Profiles of Bacteria in the Tropical and Subarctic Oceans Revealed by Pyrosequencing[J]. PLOS ONE,2013,8(11).
APA Jing, Hongmei,Xia, Xiaomin,Suzuki, Koji,&Liu, Hongbin.(2013).Vertical Profiles of Bacteria in the Tropical and Subarctic Oceans Revealed by Pyrosequencing.PLOS ONE,8(11).
MLA Jing, Hongmei,et al."Vertical Profiles of Bacteria in the Tropical and Subarctic Oceans Revealed by Pyrosequencing".PLOS ONE 8.11(2013).
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