Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Case9 System for Analyzing Magnetotactic Behavior
Chen, Haitao1,2,3; Zhang, Sheng-Da3,4,7; Chen, Linjie1,2,3; Cai, Yao5; Zhang, Wei-Jia3,4; Song, Tao1,2,3; Wu, Long-Fei3,6
2018-07-17
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume9Issue:1Pages:13
Abstract

Magnetotactic bacteria (MTB) are a diverse group of microorganisms capable of using geomagnetic fields for navigation. This magnetotactic behavior can help microorganisms move toward favorable habitats for optimal growth and reproduction. A comprehensive understanding of the magnetotactic mechanism at molecular levels requires highly efficient genomic editing tools, which remain underdeveloped in MTB. Here, we adapted an engineered CRISPR-Cas9 system for efficient inactivation of genes in a widely used MTB model strain, Magnetospirillum magneticum AMB-1. By combining a nuclease-deficient Cas9 (dCas9) and single-guide RNA (sgRNA), a CRISPR interference system was successfully developed to repress amb0994 expression. Furthermore, we constructed an in-frame deletion mutant of amb0994 by developing a CRISPR-Cas9 system. This mutant produces normal magnetosomes; however, its response to abrupt magnetic field reversals is faster than wild-type strain. This behavioral difference is probably a consequence of altered flagella function, as suggested with our dynamics simulation study by modeling M. magneticum AMB-1 cell as an ellipsoid. These data indicate that, Amb0994 is involved in the cellular response to magnetic torque changes via controlling flagella. In summary, this study, besides contributing to a better understanding of magnetotaxis mechanism, demonstrated the CRISPR-(d)Cas9 system as a useful genetic tool for efficient genome editing in MTB.

KeywordCrispr-cas9 Magnetotactic Bacteria Magnetotaxis Amb0994 Dynamics Simulation
DOI10.3389/fmicb.2018.01569
Indexed BySCI
Funding OrganizationNational Youth Foundation of China ; National Key Research and Development Program of China ; Institute of Electrical Engineering, Chinese Academy of Sciences
Language英语
Funding ProjectNational Youth Foundation of China[31300689] ; National Key Research and Development Program of China[2017YFC0108501] ; Institute of Electrical Engineering, Chinese Academy of Sciences[Y650141CSA]
WOS KeywordSequence-specific Control ; Sp Strain Amb-1 ; Magnetosome Formation ; Gene-expression ; Bacterial Chemoreceptors ; Crispr/cas9 System ; Geomagnetic-fields ; Cas System ; Cell ; Mamk
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000438923900001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/7036
Collection深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
Corresponding AuthorSong, Tao; Wu, Long-Fei
Affiliation1.Chinese Acad Sci, Inst Elect Engn, Beijing Key Lab Biol Electromagnetism, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, CNRS Marseille, Rance China Int Lab Evolut & Dev Magnetotact Mult, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Deep Sea Microbial Cell Biol, Sanya, Peoples R China
5.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing, Peoples R China
6.Aix Marseille Univ, LCB, CNRS, Marseille, France
7.Tech Univ Denmark, Dept Biotechnol & Biomed, Lyngby, Denmark
Recommended Citation
GB/T 7714
Chen, Haitao,Zhang, Sheng-Da,Chen, Linjie,et al. Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Case9 System for Analyzing Magnetotactic Behavior[J]. FRONTIERS IN MICROBIOLOGY,2018,9(1):13.
APA Chen, Haitao.,Zhang, Sheng-Da.,Chen, Linjie.,Cai, Yao.,Zhang, Wei-Jia.,...&Wu, Long-Fei.(2018).Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Case9 System for Analyzing Magnetotactic Behavior.FRONTIERS IN MICROBIOLOGY,9(1),13.
MLA Chen, Haitao,et al."Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Case9 System for Analyzing Magnetotactic Behavior".FRONTIERS IN MICROBIOLOGY 9.1(2018):13.
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