Proliferation of hydrocarbon-degrading microbes at the bottom of the Mariana Trench
Liu, Jiwen1,2; Zheng, Yanfen1; Lin, Heyu1; Wang, Xuchen3; Li, Meng4; Liu, Yang4; Yu, Meng3; Zhao, Meixun2,3; Pedentchouk, Nikolai5; Lea-Smith, David J.6; Todd, Jonathan D.6; Magill, Clayton R.7; Zhang, Wei-Jia8; Zhou, Shun1; Song, Delei1; Zhong, Haohui1; Xin, Yu2,3; Yu, Min1,2; Tian, Jiwei9,10; Zhang, Xiao-Hua1,2
2019-04-12
Source PublicationMICROBIOME
ISSN2049-2618
Volume7Pages:13
AbstractBackground: The Mariana Trench is the deepest known site in the Earth's oceans, reaching a depth of similar to 11,000m at the Challenger Deep. Recent studies reveal that hadal waters harbor distinctive microbial planktonic communities. However, the genetic potential of microbial communities within the hadal zone is poorly understood. Results: Here, implementing both culture-dependent and culture-independent methods, we perform extensive analysis of microbial populations and their genetic potential at different depths in the Mariana Trench. Unexpectedly, we observed an abrupt increase in the abundance of hydrocarbon-degrading bacteria at depths > 10,400m in the Challenger Deep. Indeed, the proportion of hydrocarbon-degrading bacteria at > 10,400m is the highest observed in any natural environment on Earth. These bacteria were mainly Oleibacter, Thalassolituus, and Alcanivorax genera, all of which include species known to consume aliphatic hydrocarbons. This community shift towards hydrocarbon degraders was accompanied by increased abundance and transcription of genes involved in alkane degradation. Correspondingly, three Alcanivorax species that were isolated from 10,400m water supplemented with hexadecane were able to efficiently degrade n-alkanes under conditions simulating the deep sea, as did a reference Oleibacter strain cultured at atmospheric pressure. Abundant n-alkanes were observed in sinking particles at 2000, 4000, and 6000m (averaged 23.5 mu g/gdw) and hadal surface sediments at depths of 10,908, 10,909, and 10,911m (averaged 2.3 mu g/gdw). The delta H-2 values of n-C-16/18 alkanes that dominated surface sediments at near 11,000-m depths ranged from -79 to -93%, suggesting that these sedimentary alkanes may have been derived from an unknown heterotrophic source. Conclusions: These results reveal that hydrocarbon-degrading microorganisms are present in great abundance in the deepest seawater on Earth and shed a new light on potential biological processes in this extreme environment.
KeywordChallenger Deep Mariana Trench Hadal water Metagenomics Microbial community Hydrocarbon degradation Hydrocarbon biosynthesis
DOI10.1186/s40168-019-0652-3
Funding OrganizationNational Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology ; National Program on Global Change and Air-Sea Interaction ; Chinese Academy of Sciences ; Natural Environmental Research (NERC)
Language英语
Funding ProjectNational Natural Science Foundation of China[91751202] ; National Natural Science Foundation of China[41730530] ; National Natural Science Foundation of China[41476112] ; National Natural Science Foundation of China[41506154] ; Fundamental Research Funds for the Central Universities[201762009] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2016ASKJ14] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2016ASKJ15] ; National Program on Global Change and Air-Sea Interaction[GASI-02-PAC-ST-MSwin] ; Chinese Academy of Sciences[XDB06010203] ; Natural Environmental Research (NERC)[NE/S001352] ; Natural Environmental Research (NERC)[NE/P012671] ; Natural Environmental Research (NERC)[NE/N002385]
WOS KeywordSINGLE CELLS ; DEEP ; COMMUNITY ; SURFACE ; WATERS ; CARBON ; ALTEROMONAS ; DIVERSITY ; BACTERIUM ; LIPIDS
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000464748100001
PublisherBMC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/6739
Collection深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
Corresponding AuthorTian, Jiwei; Zhang, Xiao-Hua
Affiliation1.Ocean Univ China, Coll Marine Life Sci, MOE Key Lab Marine Genet & Breeding, Qingdao 266003, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China
3.Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Shandong, Peoples R China
4.Shenzhen Univ, Inst Adv Study, Shenzhen 518060, Peoples R China
5.Univ East Anglia, Sch Environm Sci, Norwich NR4 7TJ, Norfolk, England
6.Univ East Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England
7.Heriot Watt Univ, Lyell Ctr, Edinburgh EH14 4AS, Midlothian, Scotland
8.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Deep Sea Microbial Cell Biol, Sanya 572000, Peoples R China
9.Ocean Univ China, Key Lab Phys Oceanog, Minist Educ, Qingdao 266100, Shandong, Peoples R China
10.Qingdao Natl Lab Marine Sci & Technol, Marine Dynam Proc & Climate Funct Lab, Qingdao 266237, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jiwen,Zheng, Yanfen,Lin, Heyu,et al. Proliferation of hydrocarbon-degrading microbes at the bottom of the Mariana Trench[J]. MICROBIOME,2019,7:13.
APA Liu, Jiwen.,Zheng, Yanfen.,Lin, Heyu.,Wang, Xuchen.,Li, Meng.,...&Zhang, Xiao-Hua.(2019).Proliferation of hydrocarbon-degrading microbes at the bottom of the Mariana Trench.MICROBIOME,7,13.
MLA Liu, Jiwen,et al."Proliferation of hydrocarbon-degrading microbes at the bottom of the Mariana Trench".MICROBIOME 7(2019):13.
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