Characteristics of a Novel Manganese Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench
Li, Yanan1,2; Kong, Xue1,2; Zhang, Haibin1
2019-02-01
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume17Issue:2Pages:12
Abstract

A novel, cold-adapted, and acid-base stable manganese superoxide dismutase (Ps-Mn-SOD) was cloned from hadal sea cucumber Paelopatides sp. The dimeric recombinant enzyme exhibited approximately 60 kDa in molecular weight, expressed activity from 0 degrees C to 70 degrees C with an optimal temperature of 0 degrees C, and resisted wide pH values from 2.2-13.0 with optimal activity (> 70%) at pH 5.0-12.0. The Km and Vmax of Ps-Mn-SOD were 0.0329 +/- 0.0040 mM and 9112 +/- 248 U/mg, respectively. At tested conditions, Ps-Mn-SOD was relatively stable in divalent metal ion and other chemicals, such as -mercaptoethanol, dithiothreitol, Tween 20, Triton X-100, and Chaps. Furthermore, the enzyme showed striking stability in 5 M urea or 4 M guanidine hydrochloride, resisted digestion by proteases, and tolerated a high hydrostatic pressure of 100 MPa. The resistance of Ps-Mn-SOD against low temperature, extreme acidity and alkalinity, chemicals, proteases, and high pressure make it a potential candidate in biopharmaceutical and nutraceutical fields.

KeywordExpression Purification Deep-sea Enzyme Pcold Vector
DOI10.3390/md17020084
Indexed BySCI
Funding OrganizationNational Key Research and Development Program of China ; Hundred Talents Program of CAS
Language英语
Funding ProjectNational Key Research and Development Program of China[2017YFC0306600] ; National Key Research and Development Program of China[2018YFC0309804] ; Hundred Talents Program of CAS[SIDSSE-BR-201401]
WOS KeywordMolecular-cloning ; Expression ; Copper ; Sod ; Purification ; Bacterium ; Japonicus ; Neurons ; Oxygen ; Yeast
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000460795500012
PublisherMDPI
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/6671
Collection深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
Corresponding AuthorZhang, Haibin
Affiliation1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Corresponding Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
Li, Yanan,Kong, Xue,Zhang, Haibin. Characteristics of a Novel Manganese Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench[J]. MARINE DRUGS,2019,17(2):12.
APA Li, Yanan,Kong, Xue,&Zhang, Haibin.(2019).Characteristics of a Novel Manganese Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench.MARINE DRUGS,17(2),12.
MLA Li, Yanan,et al."Characteristics of a Novel Manganese Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench".MARINE DRUGS 17.2(2019):12.
Files in This Item: Download All
File Name/Size DocType Version Access License
Characteristics of a(3064KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Yanan]'s Articles
[Kong, Xue]'s Articles
[Zhang, Haibin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Yanan]'s Articles
[Kong, Xue]'s Articles
[Zhang, Haibin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Yanan]'s Articles
[Kong, Xue]'s Articles
[Zhang, Haibin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Characteristics of a Novel Manganese Superoxide.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.