Hadal water sampling by in situ microbial filtration and fixation (ISMIFF) apparatus
Wang, Yong1; Gao, Zhao-Ming1; Li, Jun1; He, Li-Sheng1; Cui, Guo-Jie1,2; Li, Wen-Li1,2; Chen, Jun1,2; Xin, Yong-Zhi1; Cai, Du-Si1; Zhang, Ai-Qun1
2019-02-01
Source PublicationDEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS
ISSN0967-0637
Volume144Pages:132-137
AbstractThe deepest point of the oceans is over 10 km depth and is characterized with extremely high hydrostatic pressure. Water sampling from the hadal zone for microbiological studies is therefore difficult. In this study, we demonstrate the application of an in situ microbial filtration and fixation (ISMIFF) apparatus in sampling work from the Challenger Deep. A total of 2300 L hadal waters were filtered by the ISMIFF equipped on a hadal lander during seven deployments. The microbial communities in the hadal samples ( > 6000 m) collected by the ISMIFF were composed of more heterotrophic Marinimicrobia and less photoautotrophic Prochlorococcus and SAR11 bacteria, compared with those by Niskin bottles. Such a difference in the community structure was not observed in the samples collected by the two methods from 5000-m depth, indicating an effect of greater depths on sample quality. Our results indicate that the ISMIFF or similar filtration apparatuses are indispensable for hadal water sampling since using Niskin bottles is probably difficult to obtain pristine samples from the hadal zone. Application of the ISMIFF will pave the way to meta-omics studies on the hadal microbes.
KeywordHadal trench In situ sampling ISMIFF Microbial community
DOI10.1016/j.dsr.2019.01.009
Funding OrganizationNational Key Research and Development Program of China ; Strategic Priority Research Program B of Chinese Academy of Sciences ; National Science Foundation of China
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFC0302500] ; Strategic Priority Research Program B of Chinese Academy of Sciences[XDB06010201] ; Strategic Priority Research Program B of Chinese Academy of Sciences[XDB06010103] ; Strategic Priority Research Program B of Chinese Academy of Sciences[XDB06040100] ; National Science Foundation of China[41476104] ; National Science Foundation of China[31460001]
WOS KeywordOCEAN ; SEA ; BACTERIA ; TRENCH
WOS Research AreaOceanography
WOS SubjectOceanography
WOS IDWOS:000460831000011
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/6666
Collection深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
Corresponding AuthorWang, Yong
Affiliation1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, 28 Luhuitou Rd, Sanya, Hainan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yong,Gao, Zhao-Ming,Li, Jun,et al. Hadal water sampling by in situ microbial filtration and fixation (ISMIFF) apparatus[J]. DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS,2019,144:132-137.
APA Wang, Yong.,Gao, Zhao-Ming.,Li, Jun.,He, Li-Sheng.,Cui, Guo-Jie.,...&Zhang, Ai-Qun.(2019).Hadal water sampling by in situ microbial filtration and fixation (ISMIFF) apparatus.DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS,144,132-137.
MLA Wang, Yong,et al."Hadal water sampling by in situ microbial filtration and fixation (ISMIFF) apparatus".DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS 144(2019):132-137.
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