Pressure-Regulated Gene Expression and Enzymatic Activity o the Two Periplasmic Nitrate Reductases in the Deep-Sea Bacterium Shewanella piezotolerans WP3
Li, Xue-Gong1,2; Zhang, Wei-Jia1,2; Xiao, Xiang3,4; Jian, Hua-Hua3; Jiang, Ting1,5; Tang, Hong-Zhi1,5; Qi, Xiao-Qing1,2; Wu, Long-Fei2,6
2018-12-21
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume9Pages:9
AbstractShewanella species are widely distributed in marine environments, from the shallow coasts to the deepest sea bottom. Most Shewanella species possess two isoforms of periplasmic nitrate reductases (NAP-alpha and NAP-beta) and are able to generate energy through nitrate reduction. However, the contributions of the two NAP systems to bacterial deep-sea adaptation remain unclear. In this study, we found that the deep-sea denitrifier Shewanella piezotolerans WP3 was capable of performing nitrate respiration under high hydrostatic pressure (HHP) conditions. In the wild-type strain, NAP-beta played a dominant role and was induced by both the substrate and an elevated pressure, whereas NAP-alpha was constitutively expressed at a relatively lower level. Genetic studies showed that each NAP system alone was sufficient to fully sustain nitrate-dependent growth and that both NAP systems exhibited substrate and pressure inducible expression patterns when the other set was absent. Biochemical assays further demonstrated that NAP-alpha had a higher tolerance to elevated pressure. Collectively, we report for the first time the distinct properties and contributions of the two NAP systems to nitrate reduction under different pressure conditions. The results will shed light on the mechanisms of bacterial HHP adaptation and nitrogen cycling in the deep-sea environment.
KeywordShewanella piezotolerans WP3 nitrate reduction periplasmic nitrate reductase (NAP) high hydrostatic pressure piezotolerance
DOI10.3389/fmicb.2018.03173
Funding OrganizationNational Key R&D Program of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Sanya City ; Centre National de la Recherche Scientifique
Language英语
Funding ProjectNational Key R&D Program of China[2018YFC0309904] ; National Key R&D Program of China[2016YFC0304905] ; National Key R&D Program of China[2016YFC0302502] ; National Natural Science Foundation of China[NSFC 91751202] ; National Natural Science Foundation of China[41806174] ; National Natural Science Foundation of China[41506147] ; National Natural Science Foundation of China[91751108] ; Chinese Academy of Sciences[XDB06010203] ; Sanya City[2016PT18] ; Centre National de la Recherche Scientifique
WOS KeywordPHOTOBACTERIUM-PROFUNDUM ; PHYSIOLOGICAL ROLES ; VIOLACEA DSS12 ; ONEIDENSIS ; REDUCTION ; NAP ; METABOLISM ; PROTEIN ; NITRITE ; GROWTH
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000454073000001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/6455
Collection深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
Corresponding AuthorZhang, Wei-Jia
Affiliation1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Deep Sea Microbial Cell Biol, Sanya, Peoples R China
2.CNRS Marseille, CAS, Int Associated Lab Evolut & Dev Magnetotact Multi, Sanya, Peoples R China
3.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Sch Life Sci & Biotechnol, Shanghai, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Naval Architecture Ocean & Civil Engn, State Key Lab Ocean Engn, Shanghai, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Aix Marseille Univ, CNRS, LCB, Marseille, France
Recommended Citation
GB/T 7714
Li, Xue-Gong,Zhang, Wei-Jia,Xiao, Xiang,et al. Pressure-Regulated Gene Expression and Enzymatic Activity o the Two Periplasmic Nitrate Reductases in the Deep-Sea Bacterium Shewanella piezotolerans WP3[J]. FRONTIERS IN MICROBIOLOGY,2018,9:9.
APA Li, Xue-Gong.,Zhang, Wei-Jia.,Xiao, Xiang.,Jian, Hua-Hua.,Jiang, Ting.,...&Wu, Long-Fei.(2018).Pressure-Regulated Gene Expression and Enzymatic Activity o the Two Periplasmic Nitrate Reductases in the Deep-Sea Bacterium Shewanella piezotolerans WP3.FRONTIERS IN MICROBIOLOGY,9,9.
MLA Li, Xue-Gong,et al."Pressure-Regulated Gene Expression and Enzymatic Activity o the Two Periplasmic Nitrate Reductases in the Deep-Sea Bacterium Shewanella piezotolerans WP3".FRONTIERS IN MICROBIOLOGY 9(2018):9.
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