Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria
Yin Qunjian1,2,3,4; Zhang Weijia1,3,4,5; Li Xuegong1,3,4,5; Zhou Lihong1,3,4,5; Qi Xiaoqing1,3,4,5; Zhang Chan1,2; Wu Long-Fei3,4,6
2019
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume37Issue:1Pages:210-222
AbstractTrimethylamine N-oxide (TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from different genera harbor high hydrostatic pressure (HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from fish which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affiliated to 23 genera of Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at different depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas, Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-specific feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the first time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.
Keywordmarine bacteria Trimethylamine N-oxide (TMAO) high hydrostatic pressure (HHP) pressure tolerance phenotype
DOI10.1007/s00343-019-7377-9
Funding OrganizationNational Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Key Research and Development Program of Hainan Province ; Natural Science Foundation of Hainan Province ; Centre National de la Recherche Scientifique ; Sanya City
Language英语
Funding ProjectNational Natural Science Foundation of China[41506147] ; National Natural Science Foundation of China[91751108] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB06010203] ; Key Research and Development Program of Hainan Province[ZDYF2016211] ; Natural Science Foundation of Hainan Province[20163151] ; Centre National de la Recherche Scientifique ; Sanya City[2016PT18]
WOS KeywordHYDROSTATIC-PRESSURE ; RESPIRATORY SYSTEM ; VIBRIO-FLUVIALIS ; GENOME SEQUENCE ; MARINE FISH ; SP NOV. ; SHEWANELLA ; TMAO ; OCEAN ; WATER
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000459304100019
PublisherSCIENCE PRESS
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Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/5945
Collection深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
Corresponding AuthorZhang Weijia
Affiliation1.Chinese Acad Sci, Lab Deep Sea Microbial Cell Biol, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.CNRS Marseillel, Int Associated Lab Evolut & Dev Magnetotact Mult, Marseille, France
4.CAS Beijing Qingdao Sanya, Beijing, Peoples R China
5.Chinese Acad Sci, CAS Key Lab Expt Study Deep Sea Extreme Condit, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
6.CNRS Marseille, AMU, LCB UMR 7283, F-143402 Marseille, France
Recommended Citation
GB/T 7714
Yin Qunjian,Zhang Weijia,Li Xuegong,et al. Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(1):210-222.
APA Yin Qunjian.,Zhang Weijia.,Li Xuegong.,Zhou Lihong.,Qi Xiaoqing.,...&Wu Long-Fei.(2019).Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(1),210-222.
MLA Yin Qunjian,et al."Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.1(2019):210-222.
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