Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench
Li, Yanan1,2; Kong, Xue1,2; Chen, Jiawei1,2; Liu, Helu1; Zhang, Haibin1
2018-05-01
发表期刊MARINE DRUGS
ISSN1660-3397
卷号16期号:5页码:15
摘要Superoxide dismutases (SODs) are among the most important antioxidant enzymes and show great potential in preventing adverse effects during therapeutic trials. In the present study, cloning, expression, and characterization of a novel Cu,Zn superoxide dismutase (Ps-Cu,Zn-SOD) from a hadal sea cucumber (Paelopatides sp.) were reported. Phylogenetic analysis showed that Ps-Cu,Zn-SOD belonged to a class of intracellular SOD. Its Km and Vmax were 0.0258 +/- 0.0048 mM and 925.1816 +/- 28.0430 units/mg, respectively. The low Km value of this enzyme represents a high substrate affinity and can adapt to the low metabolic rate of deep sea organisms. The enzyme functioned from 0 degrees C to 80 degrees C with an optimal temperature of 40 degrees C. Moreover, the enzyme activity was maintained up to 87.12% at 5 degrees C. The enzyme was active at pH 4 to 12 with an optimal pH of 8.5. Furthermore, Ps-Cu,Zn-SOD tolerated high concentration of urea and GuHCl, resisted hydrolysis by proteases, and maintained stability at high pressure. All these features demonstrated that the deep sea Ps-Cu,Zn-SOD is a potential candidate for application to the biopharmaceutical field.
关键词superoxide dismutase (SOD) Paelopatides expression pressure Mariana Trench deep sea sea cucumber
DOI10.3390/md16050169
资助者Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; "Hundred Talents Program" of the Chinese Academy of Sciences
语种英语
关键词[WOS]AMYOTROPHIC-LATERAL-SCLEROSIS ; DOUBLE-BLIND ; PROTEIN ; CLONING ; BIOTECHNOLOGY ; PURIFICATION ; EXPRESSION ; GENE ; EPT3 ; SOD
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDB06010104] ; National Key Research and Development Program of China[2017YFC0306600] ; National Natural Science Foundation of China[41576127] ; "Hundred Talents Program" of the Chinese Academy of Sciences[SIDSSE-BR-201401] ; "Hundred Talents Program" of the Chinese Academy of Sciences[Y510021]
WOS研究方向Pharmacology & Pharmacy
WOS类目Chemistry, Medicinal
WOS记录号WOS:000435195100032
出版者MDPI
引用统计
文献类型期刊论文
条目标识符http://ir.idsse.ac.cn/handle/183446/5943
专题深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者Zhang, Haibin
作者单位1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100039, Peoples R China
第一作者单位中国科学院深海科学与工程研究所
通讯作者单位中国科学院深海科学与工程研究所
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Li, Yanan,Kong, Xue,Chen, Jiawei,et al. Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench[J]. MARINE DRUGS,2018,16(5):15.
APA Li, Yanan,Kong, Xue,Chen, Jiawei,Liu, Helu,&Zhang, Haibin.(2018).Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench.MARINE DRUGS,16(5),15.
MLA Li, Yanan,et al."Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench".MARINE DRUGS 16.5(2018):15.
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