Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench
Li, Yanan1,2; Kong, Xue1,2; Chen, Jiawei1,2; Liu, Helu1; Zhang, Haibin1
2018-05-01
Source PublicationMARINE DRUGS
Volume16Issue:5Pages:1
Abstract

Superoxide dismutases (SODs) are among the most important antioxidant enzymes and show great potential in preventing adverse effects during therapeutic trials. In the present study, cloning, expression, and characterization of a novel Cu,Zn superoxide dismutase (Ps-Cu,Zn-SOD) from a hadal sea cucumber (Paelopatides sp.) were reported. Phylogenetic analysis showed that Ps-Cu,Zn-SOD belonged to a class of intracellular SOD. Its Km and Vmax were 0.0258 +/- 0.0048 mM and 925.1816 +/- 28.0430 units/mg, respectively. The low Km value of this enzyme represents a high substrate affinity and can adapt to the low metabolic rate of deep sea organisms. The enzyme functioned from 0 degrees C to 80 degrees C with an optimal temperature of 40 degrees C. Moreover, the enzyme activity was maintained up to 87.12% at 5 degrees C. The enzyme was active at pH 4 to 12 with an optimal pH of 8.5. Furthermore, Ps-Cu,Zn-SOD tolerated high concentration of urea and GuHCl, resisted hydrolysis by proteases, and maintained stability at high pressure. All these features demonstrated that the deep sea Ps-Cu,Zn-SOD is a potential candidate for application to the biopharmaceutical field.

SubtypeArticle
KeywordSuperoxide Dismutase (Sod) Paelopatides Expression Pressure Mariana Trench Deep Sea Sea Cucumber
Subject Area生物学 ; 生物化学 ; 生理学
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.3390/md16050169
URL查看原文
Indexed BySCI
Language英语
WOS KeywordAmyotrophic-lateral-sclerosis ; Double-blind ; Protein ; Cloning ; Biotechnology ; Purification ; Expression ; Gene ; Ept3 ; Sod
WOS SubjectChemistry, Medicinal
WOS IDWOS:000435195100032
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/5943
Collection深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
Corresponding AuthorZhang, Haibin
Affiliation1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Corresponding Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
Li, Yanan,Kong, Xue,Chen, Jiawei,et al. Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench[J]. MARINE DRUGS,2018,16(5):1.
APA Li, Yanan,Kong, Xue,Chen, Jiawei,Liu, Helu,&Zhang, Haibin.(2018).Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench.MARINE DRUGS,16(5),1.
MLA Li, Yanan,et al."Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench".MARINE DRUGS 16.5(2018):1.
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