Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench
Li, Yanan1,2; Kong, Xue1,2; Chen, Jiawei1,2; Liu, Helu1; Zhang, Haibin1; Haibin Zhang; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
2018-05-01
发表期刊MARINE DRUGS
卷号16期号:5
产权排序第1完成单位 ; 第2完成单位 ; 第1完成单位 ; 第2完成单位
摘要Superoxide dismutases (SODs) are among the most important antioxidant enzymes and show great potential in preventing adverse effects during therapeutic trials. In the present study, cloning, expression, and characterization of a novel Cu,Zn superoxide dismutase (Ps-Cu,Zn-SOD) from a hadal sea cucumber (Paelopatides sp.) were reported. Phylogenetic analysis showed that Ps-Cu,Zn-SOD belonged to a class of intracellular SOD. Its Km and Vmax were 0.0258 +/- 0.0048 mM and 925.1816 +/- 28.0430 units/mg, respectively. The low Km value of this enzyme represents a high substrate affinity and can adapt to the low metabolic rate of deep sea organisms. The enzyme functioned from 0 degrees C to 80 degrees C with an optimal temperature of 40 degrees C. Moreover, the enzyme activity was maintained up to 87.12% at 5 degrees C. The enzyme was active at pH 4 to 12 with an optimal pH of 8.5. Furthermore, Ps-Cu,Zn-SOD tolerated high concentration of urea and GuHCl, resisted hydrolysis by proteases, and maintained stability at high pressure. All these features demonstrated that the deep sea Ps-Cu,Zn-SOD is a potential candidate for application to the biopharmaceutical field.
文章类型Article
关键词Superoxide Dismutase (Sod) Paelopatides Expression Pressure Mariana Trench Deep Sea Sea Cucumber
学科领域生物学 ; 生物化学 ; 生理学
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.3390/md16050169
URL查看原文 ; 查看原文
收录类别SCI
语种英语
关键词[WOS]AMYOTROPHIC-LATERAL-SCLEROSIS ; DOUBLE-BLIND ; PROTEIN ; CLONING ; BIOTECHNOLOGY ; PURIFICATION ; EXPRESSION ; GENE ; EPT3 ; SOD
WOS类目Chemistry, Medicinal
WOS记录号WOS:000435195100032
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文献类型期刊论文
版本出版稿
条目标识符http://ir.idsse.ac.cn/handle/183446/5943
专题深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者Zhang, Haibin; Haibin Zhang; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
作者单位1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100039, Peoples R China
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Li, Yanan,Kong, Xue,Chen, Jiawei,et al. Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench[J]. MARINE DRUGS,2018,16(5).
APA Li, Yanan.,Kong, Xue.,Chen, Jiawei.,Liu, Helu.,Zhang, Haibin.,...&Zhang, Haibin.(2018).Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench.MARINE DRUGS,16(5).
MLA Li, Yanan,et al."Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench".MARINE DRUGS 16.5(2018).
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