De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids
Liu, Helu; Kong, Xue; Chen, Jiawei; Zhang, Haibin; Haibin Zhang; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
2018-04-01
Source PublicationAQUACULTURE
Volume491Pages:358-367
Contribution Rank第1完成单位
AbstractAs the widespread species in the tropical Indo-Pacific area, the sea cucumber Stichopus horrens is characterized by the presence of appreciable amounts of triterpene glycosides. However, genes involved in biosynthesis of the triterpenoids in sea cucumber have rarely been researched. Here, we aim to explore the triterpenoid biosynthesis of S. horrens collected in the south sea of China. In this study, the de novo sequencing of S. horrens was carried out by Illumina HiSeq 2000. A total of 99,854,932 high quality sequence reads were obtained. These raw data were assembled into 168,151 contigs, and 78,024 unigenes were left after redundance removing. The transcriptomic data clearly showed that triterpenoid biosynthesis was via the MVA pathway in S. horrens. The content of total triterpene glycosides was examined by method of orcinol reaction, and 0.116 +/- 0.012 mg/g and 0.016 +/- 0.003 mg/g in intestine and body wall, respectively. qRTPCR showed that both oxidosqualene cyclases OSC1 and OSC2 are highly expressed in intestine, suggesting their commitment to triterpenoid biosynthesis. We also reported some potential genes encoding Cytochrome P450 and glycosylations enzymes, which might be involved in the late step of biosynthesis of triterpenoid glycosides. Overall, the present study provided the basic data for future molecular studies on biosynthesis of triterpene glycosides in sea cucumber.
SubtypeArticle
KeywordSea Cucumber Triterpene Glycosides Transcriptome Biosynthesis Cyclase
Subject Area生物化学 ; 细胞生物学 ; 分子生物学
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.aquaculture.2018.01.012
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSPONGE ECTYOPLASIA-FEROX ; SEA-CUCUMBERS ; BIOLOGICAL-ACTIVITIES ; MARINE-INVERTEBRATES ; GLYCOSIDES ; SAPONINS ; ECHINODERMATA ; STARFISH ; GINSENG
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000431009400046
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/5942
Collection深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
Corresponding AuthorZhang, Haibin; Haibin Zhang; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
AffiliationChinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Corresponding Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
Liu, Helu,Kong, Xue,Chen, Jiawei,et al. De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids[J]. AQUACULTURE,2018,491:358-367.
APA Liu, Helu,Kong, Xue,Chen, Jiawei,Zhang, Haibin,Haibin Zhang,&Zhang, Haibin.(2018).De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids.AQUACULTURE,491,358-367.
MLA Liu, Helu,et al."De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids".AQUACULTURE 491(2018):358-367.
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