De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids
Liu, Helu; Kong, Xue; Chen, Jiawei; Zhang, Haibin; Haibin Zhang; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
2018-04-01
发表期刊AQUACULTURE
卷号491页码:358-367
产权排序第1完成单位
摘要As the widespread species in the tropical Indo-Pacific area, the sea cucumber Stichopus horrens is characterized by the presence of appreciable amounts of triterpene glycosides. However, genes involved in biosynthesis of the triterpenoids in sea cucumber have rarely been researched. Here, we aim to explore the triterpenoid biosynthesis of S. horrens collected in the south sea of China. In this study, the de novo sequencing of S. horrens was carried out by Illumina HiSeq 2000. A total of 99,854,932 high quality sequence reads were obtained. These raw data were assembled into 168,151 contigs, and 78,024 unigenes were left after redundance removing. The transcriptomic data clearly showed that triterpenoid biosynthesis was via the MVA pathway in S. horrens. The content of total triterpene glycosides was examined by method of orcinol reaction, and 0.116 +/- 0.012 mg/g and 0.016 +/- 0.003 mg/g in intestine and body wall, respectively. qRTPCR showed that both oxidosqualene cyclases OSC1 and OSC2 are highly expressed in intestine, suggesting their commitment to triterpenoid biosynthesis. We also reported some potential genes encoding Cytochrome P450 and glycosylations enzymes, which might be involved in the late step of biosynthesis of triterpenoid glycosides. Overall, the present study provided the basic data for future molecular studies on biosynthesis of triterpene glycosides in sea cucumber.
文章类型Article
关键词Sea Cucumber Triterpene Glycosides Transcriptome Biosynthesis Cyclase
学科领域生物化学 ; 细胞生物学 ; 分子生物学
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.aquaculture.2018.01.012
URL查看原文
收录类别SCI
语种英语
关键词[WOS]SPONGE ECTYOPLASIA-FEROX ; SEA-CUCUMBERS ; BIOLOGICAL-ACTIVITIES ; MARINE-INVERTEBRATES ; GLYCOSIDES ; SAPONINS ; ECHINODERMATA ; STARFISH ; GINSENG
WOS类目Fisheries ; Marine & Freshwater Biology
WOS记录号WOS:000431009400046
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文献类型期刊论文
版本出版稿
条目标识符http://ir.idsse.ac.cn/handle/183446/5942
专题深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者Zhang, Haibin; Haibin Zhang; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
作者单位Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
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Liu, Helu,Kong, Xue,Chen, Jiawei,et al. De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids[J]. AQUACULTURE,2018,491:358-367.
APA Liu, Helu,Kong, Xue,Chen, Jiawei,Zhang, Haibin,Haibin Zhang,&Zhang, Haibin.(2018).De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids.AQUACULTURE,491,358-367.
MLA Liu, Helu,et al."De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids".AQUACULTURE 491(2018):358-367.
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