DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae)
Liu, Jun; Zhang, Haibin
2018
Source PublicationMITOCHONDRIAL DNA PART A
ISSN2470-1394
Volume29Issue:8Pages:1165-1173
Abstract

Deep-sea clams (Bivalvia: Vesicomyidae) have been found in reduced environments over the world oceans, but taxonomy of this group remains confusing at species and supraspecific levels due to their high-morphological similarity and plasticity. In the present study, we collected mitochondrial COI sequences to evaluate the utility of DNA barcoding on identifying vesicomyid species. COI dataset identified 56 well-supported putative species/operational taxonomic units (OTUs), approximately covering half of the extant vesicomyid species. One species (OTU2) was first detected, and may represent a new species. Average distances between species ranged from 1.65 to 29.64%, generally higher than average intraspecific distances (0-1.41%) when excluding Pliocardia sp.10 cf. venusta (average intraspecific distance 1.91%). Local barcoding gap existed in 33 of the 35 species when comparing distances of maximum interspecific and minimum interspecific distances with two exceptions (Abyssogena southwardae and Calyptogena rectimargo-starobogatovi). The barcode index number (BIN) system determined 41 of the 56 species/OTUs, each with a unique BIN, indicating their validity. Three species were found to have two BINs, together with their high level of intraspecific variation, implying cryptic diversity within them. Although fewer 16S sequences were collected, similar results were obtained. Nineteen putative species were determined and no overlap observed between intra- and inter-specific variation. Implications of DNA barcoding for the Vesicomyidae taxonomy were then discussed. Findings of this study will provide important evidence for taxonomic revision in this problematic clam group, and accelerate the discovery of new vesicomyid species in the future.

KeywordCoi Deep-sea Clams Dna Barcoding Molecular Taxonomy Vesicomyidae
DOI10.1080/24701394.2018.1424843
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences [CAS] ; Knowledge Innovation Program of CAS ; Hundred Talents Program of CAS ; Major scientific and technological projects of Hainan Province, China
Language英语
Funding ProjectNational Natural Science Foundation of China[41576127] ; Strategic Priority Research Program of the Chinese Academy of Sciences [CAS][XDB06010104] ; Knowledge Innovation Program of CAS[SIDSSE-201401] ; Hundred Talents Program of CAS[SIDSSE-BR-201401] ; Major scientific and technological projects of Hainan Province, China[ZDKJ2016009]
WOS Keyword16s Ribosomal-rna ; Oxidase Subunit-i ; Phylogenetic-relationships ; Evolutionary Relationships ; Nucleotide-sequences ; Family Vesicomyidae ; Maximum-likelihood ; Mitochondrial-dna ; Hydrothermal Vent ; Gene
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000450275000004
PublisherTAYLOR & FRANCIS LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/5941
Collection深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
Corresponding AuthorZhang, Haibin
AffiliationChinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 57200, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jun,Zhang, Haibin. DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae)[J]. MITOCHONDRIAL DNA PART A,2018,29(8):1165-1173.
APA Liu, Jun,&Zhang, Haibin.(2018).DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae).MITOCHONDRIAL DNA PART A,29(8),1165-1173.
MLA Liu, Jun,et al."DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae)".MITOCHONDRIAL DNA PART A 29.8(2018):1165-1173.
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