Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite
He, Li-Sheng1,2; Zhang, Gen2; Wang, Yong1,2; Yan, Guo-Yong1; Qian, Pei-Yuan2; Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China)
2018
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume495Issue:1Pages:969-975
Contribution Rank第1完成单位 ; 第2完成单位
AbstractBarnacles, as major fouling organisms, have attracted more attentions. It is no doubt that the study on cement proteins is required to illustrate the mechanism of barnacle cementing. A cement protein defined as Aa-cp100k was characterized from Amphibalanus amphitrite in this study. The amino acid sequence of Aa-cp100k was shown a high similarity to other three barnacles including Megabalanus rosa (Mr-cp100k), Tetraclita japonica formosana (Tj-cp100k) and Pollicipes pollicipes (Pp-cp100k). Moreover, the localization of Aa-cp100k in the vacuoles of cyprid beta secretory cells and the adult cement gland cells by immunofluorescence microscopy, indicating that Aa-cp100k existed in both cyprid and adult barnacle. Aa-cp100k from basal plate could be dissolved in urea buffer without high concentration of dithiothreitol (DTT), different from that in Megabalanus rosa, implying diverse possible roles of cp100k in cementing. (C) 2017 Published by Elsevier Inc.
SubtypeArticle
KeywordBarnacle Cement Protein Cp100k Evolution Settlement
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.bbrc.2017.11.101
URL查看原文
Indexed BySCI
Language英语
WOS KeywordBALANUS-AMPHITRITE ; UNDERWATER ADHESIVE ; MEGABALANUS-ROSA ; SERINE-PROTEASE ; CYPRIS LARVA ; IN-SITU ; PROTEINS ; BALANOIDES ; EXOCYTOSIS ; MICROSCOPY
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000423897600146
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/5926
Collection深海科学研究部_深海生物学研究室_深海无脊椎动物蛋白质组学及分子生物学研究组
Corresponding AuthorQian, Pei-Yuan; Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China)
Affiliation1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China
2.Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
He, Li-Sheng,Zhang, Gen,Wang, Yong,et al. Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,495(1):969-975.
APA He, Li-Sheng,Zhang, Gen,Wang, Yong,Yan, Guo-Yong,Qian, Pei-Yuan,&Qian, Pei-Yuan.(2018).Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495(1),969-975.
MLA He, Li-Sheng,et al."Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495.1(2018):969-975.
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