High Hydrostatic Pressure Inducible Trimethylamine N-Oxide Reductase Improves the Pressure Tolerance of Piezosensitive Bacteria Vibrio fluvialis
Yin, Qun-Jian1,2,3; Zhang, Wei-Jia1,3,4; Qi, Xiao-Qing1,3; Zhang, Sheng-Da1; Jiang, Ting1,2; Li, Xue-Gong1,3,4; Chen, Ying5; Santini, Claire-Lise3,6; Zhou, Hao7; Chou, I-Ming4,5; Wu, Long-Fei3,6; Zhang, Wei-Jia(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Deep Sea Microbial Cell Biol, Sanya, Peoples R China)
2018-01-09
Source PublicationFRONTIERS IN MICROBIOLOGY
Volume8
Contribution Rank第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位 ; 第5完成单位 ; 第6完成单位 ; 第7完成单位
AbstractHigh hydrostatic pressure (HHP) exerts severe effects on cellular processes including impaired cell division, abolished motility and affected enzymatic activities. Transcriptomic and proteomic analyses showed that bacteria switch the expression of genes involved in multiple energy metabolism pathways to cope with HHP. We sought evidence of a changing bacterial metabolism by supplying appropriate substrates that might have beneficial effects on the bacterial lifestyle at elevated pressure. We isolated a piezosensitive marine bacterium Vibrio fluvialis strain QY27 from the South China Sea. When trimethylamine N-oxide (TMAO) was used as an electron acceptor for energy metabolism, QY27 exhibited a piezophilic-like phenotype with an optimal growth at 30 MPa. Raman spectrometry and biochemistry analyses revealed that both the efficiency of the TMAO metabolism and the activity of the TMAO reductase increased under high pressure conditions. Among the two genes coding for TMAO reductase catalytic subunits, the expression level and enzymatic activity of TorA was up-regulated by elevated pressure. Furthermore, a genetic interference assay with the CRISPR-dCas9 system demonstrated that TorA is essential for underpinning the improved pressure tolerance of QY27. We extended the study to Vibrio fluvialis type strain ATCC33809 and observed the same phenotype of TMAO-metabolism improved the pressure tolerance. These results provide compelling evidence for the determinant role of metabolism in the adaption of bacteria to the deep-sea ecosystems with HHP.
SubtypeArticle
KeywordHigh Hydrostatic Pressure (Hhp) Trimethylamine N-oxide (Tmao) Pressure Tolerance Vibrio Fluvialis Raman Spectrometry Crispri
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.3389/fmicb.2017.02646
URL查看原文
Indexed BySCI
Language英语
WOS KeywordESCHERICHIA-COLI ; SHEWANELLA-VIOLACEA ; RESPIRATORY SYSTEM ; RESPONSE REGULATOR ; OPERON EXPRESSION ; GENE-EXPRESSION ; SEA ; TORR ; BINDING ; TMAO
WOS SubjectMicrobiology
WOS IDWOS:000419566800001
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/5280
Collection深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
Corresponding AuthorZhang, Wei-Jia; Zhang, Wei-Jia(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Deep Sea Microbial Cell Biol, Sanya, Peoples R China)
Affiliation1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Deep Sea Microbial Cell Biol, Sanya, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, CNRS Marseille, Int Associated Lab Evolut & Dev Magnetotact Multi, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, CAS Key Lab Expt Study Deep Sea Extreme Condit, Sanya, Peoples R China
5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Expt Study Deep Sea Extreme Condit, Sanya, Peoples R China
6.Aix Marseille Univ, CNRS Marseille, UMR 7283, LCB, Marseille, France
7.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Engn Dept, Engn Lab, Sanya, Peoples R China
Recommended Citation
GB/T 7714
Yin, Qun-Jian,Zhang, Wei-Jia,Qi, Xiao-Qing,et al. High Hydrostatic Pressure Inducible Trimethylamine N-Oxide Reductase Improves the Pressure Tolerance of Piezosensitive Bacteria Vibrio fluvialis[J]. FRONTIERS IN MICROBIOLOGY,2018,8.
APA Yin, Qun-Jian.,Zhang, Wei-Jia.,Qi, Xiao-Qing.,Zhang, Sheng-Da.,Jiang, Ting.,...&Zhang, Wei-Jia.(2018).High Hydrostatic Pressure Inducible Trimethylamine N-Oxide Reductase Improves the Pressure Tolerance of Piezosensitive Bacteria Vibrio fluvialis.FRONTIERS IN MICROBIOLOGY,8.
MLA Yin, Qun-Jian,et al."High Hydrostatic Pressure Inducible Trimethylamine N-Oxide Reductase Improves the Pressure Tolerance of Piezosensitive Bacteria Vibrio fluvialis".FRONTIERS IN MICROBIOLOGY 8(2018).
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