Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
Yan, Guoyong1,2; Zhang, Gen3; Huang, Jiaomei1; Lan, Yi4; Sun, Jin4; Zeng, Cong1; Wang, Yong1; Qian, Pei-Yuan4; He, Lisheng1; He, Lisheng(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China)
2017-11-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume18Issue:11
Contribution Rank第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位
AbstractMegabalanus barnacle is one of the model organisms for marine biofouling research. However, further elucidation of molecular mechanisms underlying larval settlement has been hindered due to the lack of genomic information thus far. In the present study, cDNA libraries were constructed for cyprids, the key stage for larval settlement, and adults of Megabalanus volcano. After high-throughput sequencing and de novo assembly, 42,620 unigenes were obtained with a N50 value of 1532 bp. These unigenes were annotated by blasting against the NCBI non-redundant (nr), Swiss-Prot, Cluster of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Finally, 19,522, 15,691, 14,459, and 10,914 unigenes were identified correspondingly. There were 22,158 differentially expressed genes (DEGs) identified between two stages. Compared with the cyprid stage, 8241 unigenes were down-regulated and 13,917 unigenes were up-regulated at the adult stage. The neuroactive ligand-receptor interaction pathway (ko04080) was significantly enriched by KEGG enrichment analysis of the DEGs, suggesting that it possibly involved in larval settlement. Potential functions of three conserved allatostatin neuropeptide-receptor pairs and two light-sensitive opsin proteins were further characterized, indicating that they might regulate attachment and metamorphosis at cyprid stage. These results provided a deeper insight into the molecular mechanisms underlying larval settlement of barnacles.
SubtypeArticle
KeywordMegabalanus Volcano Transcriptome Larval Settlement Neuropeptide Opsin Gpcrs
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.3390/ijms18112253
URL查看原文
Indexed BySCI
Language英语
WOS KeywordDIEL VERTICAL MIGRATION ; SEX PEPTIDE RECEPTOR ; BALANUS-AMPHITRITE ; JUVENILE-HORMONE ; RNA-SEQ ; FUNCTIONAL-CHARACTERIZATION ; GREGARIOUS SETTLEMENT ; RHODNIUS-PROLIXUS ; METHYL FARNESOATE ; ACORN BARNACLES
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000416811300027
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/5270
Collection深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
Corresponding AuthorHe, Lisheng; He, Lisheng(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China)
Affiliation1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100864, Peoples R China
3.Shenzhen Nobel Sci & Technol Serv Co Ltd, Shenzhen 440305, Peoples R China
4.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Yan, Guoyong,Zhang, Gen,Huang, Jiaomei,et al. Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(11).
APA Yan, Guoyong.,Zhang, Gen.,Huang, Jiaomei.,Lan, Yi.,Sun, Jin.,...&He, Lisheng.(2017).Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(11).
MLA Yan, Guoyong,et al."Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.11(2017).
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