Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
Yan, Guoyong1,2; Zhang, Gen3; Huang, Jiaomei1; Lan, Yi4; Sun, Jin4; Zeng, Cong1; Wang, Yong1; Qian, Pei-Yuan4; He, Lisheng1; He, Lisheng(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China)
2017-11-01
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号18期号:11
产权排序第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位
摘要Megabalanus barnacle is one of the model organisms for marine biofouling research. However, further elucidation of molecular mechanisms underlying larval settlement has been hindered due to the lack of genomic information thus far. In the present study, cDNA libraries were constructed for cyprids, the key stage for larval settlement, and adults of Megabalanus volcano. After high-throughput sequencing and de novo assembly, 42,620 unigenes were obtained with a N50 value of 1532 bp. These unigenes were annotated by blasting against the NCBI non-redundant (nr), Swiss-Prot, Cluster of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Finally, 19,522, 15,691, 14,459, and 10,914 unigenes were identified correspondingly. There were 22,158 differentially expressed genes (DEGs) identified between two stages. Compared with the cyprid stage, 8241 unigenes were down-regulated and 13,917 unigenes were up-regulated at the adult stage. The neuroactive ligand-receptor interaction pathway (ko04080) was significantly enriched by KEGG enrichment analysis of the DEGs, suggesting that it possibly involved in larval settlement. Potential functions of three conserved allatostatin neuropeptide-receptor pairs and two light-sensitive opsin proteins were further characterized, indicating that they might regulate attachment and metamorphosis at cyprid stage. These results provided a deeper insight into the molecular mechanisms underlying larval settlement of barnacles.
文章类型Article
关键词Megabalanus Volcano Transcriptome Larval Settlement Neuropeptide Opsin Gpcrs
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.3390/ijms18112253
URL查看原文
收录类别SCI
语种英语
关键词[WOS]DIEL VERTICAL MIGRATION ; SEX PEPTIDE RECEPTOR ; BALANUS-AMPHITRITE ; JUVENILE-HORMONE ; RNA-SEQ ; FUNCTIONAL-CHARACTERIZATION ; GREGARIOUS SETTLEMENT ; RHODNIUS-PROLIXUS ; METHYL FARNESOATE ; ACORN BARNACLES
WOS类目Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号WOS:000416811300027
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.idsse.ac.cn/handle/183446/5270
专题深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
通讯作者He, Lisheng; He, Lisheng(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China)
作者单位1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100864, Peoples R China
3.Shenzhen Nobel Sci & Technol Serv Co Ltd, Shenzhen 440305, Peoples R China
4.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China
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Yan, Guoyong,Zhang, Gen,Huang, Jiaomei,et al. Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(11).
APA Yan, Guoyong.,Zhang, Gen.,Huang, Jiaomei.,Lan, Yi.,Sun, Jin.,...&He, Lisheng.(2017).Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(11).
MLA Yan, Guoyong,et al."Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.11(2017).
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