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Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy
Gan, Qinhua1,2; Wang, Xuetao3; Wang, Yun4; Xie, Zhenyu1; Tian, Yang5; Lu, Yandu1,6; Lu, Yandu(Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China)
2017-11-01
Source PublicationADVANCED SCIENCE
Volume4Issue:11Pages:1
Abstract

The rapid and sensitive identification of invasive plant pathogens has important applications in biotechnology, plant quarantine, and food security. Current methods are far too time-consuming and need a pre-enrichment period ranging from hours to days. Here, a micro-Raman spectroscopy-based bioassay for culture-free pathogen quarantine inspection at the single cell level within 40 min is presented. The application of this approach can readily and specifically detect plant pathogens Burkholderia gladioli pv. alliicola and Erwinia chrysanthemi that are closely related pathogenically. Furthermore, the single-bacterium detection was able to discriminate them from a reference Raman spectral library including multiple quarantine-relevant pathogens with broad host ranges and an array of pathogenic variants. To show the usefulness of this assay, Burkholderia gladioli pv. alliicola and Erwinia chrysanthemi are detected at single-bacterium level in plant tissue lesions without pre-enrichment. The results are confirmed by the plate-counting method and a genetic molecular approach, which display comparable recognition ratios to the Raman spectroscopy-based bioassay. The results represent a critical step toward the use of micro-Raman spectroscopy in rapid and culture-free discrimination of quarantine relevant plant pathogens.

SubtypeArticle
KeywordCrop Pathogens Culture-free Detection Micro-raman Spectroscopy Single Cells
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1002/advs.201700127
URL查看原文
Indexed BySCI
Language英语
WOS KeywordRapid Identification ; Bacteria ; Microspectroscopy ; Microorganisms ; Capture ; Threat ; Blood ; Spp.
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000416155900004
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/5267
Collection文献信息中心
Corresponding AuthorLu, Yandu; Lu, Yandu(Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China)
Affiliation1.Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China
2.Shandong Entry Exit Inspect & Quarantine Bur, Inspect & Quarantine Technol Ctr, Qingdao 266002, Shandong, Peoples R China
3.Hisense Co, Qingdao 266555, Shandong, Peoples R China
4.Shanghai Hesen Biotech Co LTD, Shanghai 201802, Peoples R China
5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
6.Hainan Univ, Minist Educ, Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China
Recommended Citation
GB/T 7714
Gan, Qinhua,Wang, Xuetao,Wang, Yun,et al. Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy[J]. ADVANCED SCIENCE,2017,4(11):1.
APA Gan, Qinhua.,Wang, Xuetao.,Wang, Yun.,Xie, Zhenyu.,Tian, Yang.,...&Lu, Yandu.(2017).Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy.ADVANCED SCIENCE,4(11),1.
MLA Gan, Qinhua,et al."Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy".ADVANCED SCIENCE 4.11(2017):1.
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