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Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy
Gan, Qinhua1,2; Wang, Xuetao3; Wang, Yun4; Xie, Zhenyu1; Tian, Yang5; Lu, Yandu1,6; Lu, Yandu(Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China)
2017-11-01
发表期刊ADVANCED SCIENCE
卷号4期号:11
产权排序第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位 ; 第5完成单位 ; 第6完成单位
摘要The rapid and sensitive identification of invasive plant pathogens has important applications in biotechnology, plant quarantine, and food security. Current methods are far too time-consuming and need a pre-enrichment period ranging from hours to days. Here, a micro-Raman spectroscopy-based bioassay for culture-free pathogen quarantine inspection at the single cell level within 40 min is presented. The application of this approach can readily and specifically detect plant pathogens Burkholderia gladioli pv. alliicola and Erwinia chrysanthemi that are closely related pathogenically. Furthermore, the single-bacterium detection was able to discriminate them from a reference Raman spectral library including multiple quarantine-relevant pathogens with broad host ranges and an array of pathogenic variants. To show the usefulness of this assay, Burkholderia gladioli pv. alliicola and Erwinia chrysanthemi are detected at single-bacterium level in plant tissue lesions without pre-enrichment. The results are confirmed by the plate-counting method and a genetic molecular approach, which display comparable recognition ratios to the Raman spectroscopy-based bioassay. The results represent a critical step toward the use of micro-Raman spectroscopy in rapid and culture-free discrimination of quarantine relevant plant pathogens.
文章类型Article
关键词Crop Pathogens Culture-free Detection Micro-raman Spectroscopy Single Cells
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1002/advs.201700127
URL查看原文
收录类别SCI
语种英语
关键词[WOS]RAPID IDENTIFICATION ; BACTERIA ; MICROSPECTROSCOPY ; MICROORGANISMS ; CAPTURE ; THREAT ; BLOOD ; SPP.
WOS类目Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号WOS:000416155900004
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.idsse.ac.cn/handle/183446/5267
专题所领导_其他
通讯作者Lu, Yandu; Lu, Yandu(Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China)
作者单位1.Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China
2.Shandong Entry Exit Inspect & Quarantine Bur, Inspect & Quarantine Technol Ctr, Qingdao 266002, Shandong, Peoples R China
3.Hisense Co, Qingdao 266555, Shandong, Peoples R China
4.Shanghai Hesen Biotech Co LTD, Shanghai 201802, Peoples R China
5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
6.Hainan Univ, Minist Educ, Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China
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GB/T 7714
Gan, Qinhua,Wang, Xuetao,Wang, Yun,et al. Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy[J]. ADVANCED SCIENCE,2017,4(11).
APA Gan, Qinhua.,Wang, Xuetao.,Wang, Yun.,Xie, Zhenyu.,Tian, Yang.,...&Lu, Yandu.(2017).Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy.ADVANCED SCIENCE,4(11).
MLA Gan, Qinhua,et al."Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy".ADVANCED SCIENCE 4.11(2017).
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