Cloning and expression analysis of tps, and cryopreservation research of trehalose from Antarctic strain Pseudozyma sp
Yin, Hua1; Wang, Yibin2,3; He, Yingying2; Xing, Lei1; Zhang, Xiufang4; Wang, Shuai2,5; Qi, Xiaoqing2,6; Zheng, Zhou2,3; Lu, Jian7; Miao, Jinlai2,3; Miao, Jinlai(State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China)
2017-09-22
发表期刊3 BIOTECH
卷号7
产权排序第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位 ; 第5完成单位 ; 第6完成单位 ; 第7完成单位
摘要Trehalose is a non-reducing disaccharide sugar that widely exists in a variety of organisms, such as bacteria and eukaryotes except the vertebrates. It plays an important role in a number of critical metabolic functions especially in response to stressful environmental conditions. However, the biosynthetic pathways of trehalose in cold-adapted yeast and its responses to temperature and salinity changes remain little understood. In this study, the genome of Antarctic-isolated Pseudozyma sp. NJ7 was generated from which we identified the gene coding for trehalose phosphate synthase (TPS1) and trehalose phosphate phosphatase (TPS2), the two enzymes most critical for trehalose production. The whole draft genome length of Pseudozyma sp. NJ7 was 18,021,233 bp, and encoded at least 34 rRNA operons and 72 tRNAs. The open reading frame of tps1 contained 1827 nucleotide encoding 608 amino acids with a molecular weight of 67.64 kDa, and an isoelectric point of 5.54, while tps2 contained 3948 nucleotide encoding 1315 amino acids with a molecular weight of 144.47 kDa and an isoelectric point of 6.36. The TPS1 and TPS2 protein sequences were highly homologous to Moesziomyces antarcticus T-34, but TPS2 had obvious specificity and differently with others which suggest species specificity and different evolutionary history. Expression level of tps1 gene was strongly influenced by temperature and high salinity. In addition, addition of 0.5% trehalose preserved yeast cells in the short term but was not effective for cryopreservation for more than 5 days, but still suggesting that exogenous trehalose could indeed significantly improve the survival of yeast cells under freezing conditions. Our results provided new insights on the molecular basis of cold adaptations of Antarctic Pseudozyma sp., and also generated new information on the roles trehalose play in yeast tolerance to extreme conditions in the extreme Antarctic environments.
文章类型Article
关键词Trehalose Antarctic Pseudozyma Sp. Genome Trehalose Phosphate Synthase
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s13205-017-0983-3
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收录类别SCI
语种英语
关键词[WOS]TREHALOSE-6-PHOSPHATE SYNTHASE GENE ; SACCHAROMYCES-CEREVISIAE ; FREEZE TOLERANCE ; BAKERS-YEAST ; TEMPERATURES ; BIOSYNTHESIS ; 6-PHOSPHATE ; METABOLISM ; SEQUENCE ; PATHWAY
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000411736100001
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文献类型期刊论文
条目标识符http://ir.idsse.ac.cn/handle/183446/4771
专题深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
通讯作者Miao, Jinlai; Miao, Jinlai(State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China)
作者单位1.Tsingtao Brewery Co Ltd, State Key Lab Biol Fermentat Engn Beer, Qingdao 266061, Peoples R China
2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266235, Peoples R China
4.Qingdao Hiser Med Ctr, Clin Lab, Qingdao 266033, Peoples R China
5.Marine & Fisheries Monitoring Ctr Sanya, Sanya 572000, Peoples R China
6.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
7.Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China
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Yin, Hua,Wang, Yibin,He, Yingying,et al. Cloning and expression analysis of tps, and cryopreservation research of trehalose from Antarctic strain Pseudozyma sp[J]. 3 BIOTECH,2017,7.
APA Yin, Hua.,Wang, Yibin.,He, Yingying.,Xing, Lei.,Zhang, Xiufang.,...&Miao, Jinlai.(2017).Cloning and expression analysis of tps, and cryopreservation research of trehalose from Antarctic strain Pseudozyma sp.3 BIOTECH,7.
MLA Yin, Hua,et al."Cloning and expression analysis of tps, and cryopreservation research of trehalose from Antarctic strain Pseudozyma sp".3 BIOTECH 7(2017).
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