Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas
Lan, Yi1; Sun, Jin1; Tian, Renmao1; Bartlett, Douglas H.2; Li, Runsheng3; Wong, Yue Him1; Zhang, Weipeng1; Qiu, Jian-Wen3; Xu, Ting3; He, Li-Sheng4; Tabata, Harry G.1; Qian, Pei-Yuan1; Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China)
2017-07-01
Source PublicationMOLECULAR ECOLOGY
Volume26Issue:14Pages:3732-3743
Contribution Rank第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位
AbstractThe Challenger Deep in the Mariana Trench is the deepest point in the oceans of our planet. Understanding how animals adapt to this harsh environment characterized by high hydrostatic pressure, food limitation, dark and cold is of great scientific interest. Of the animals dwelling in the Challenger Deep, amphipods have been captured using baited traps. In this study, we sequenced the transcriptome of the amphipod Hirondellea gigas collected at a depth of 10,929 m from the East Pond of the Challenger Deep. Assembly of these sequences resulted in 133,041 contigs and 22,046 translated proteins. Functional annotation of these contigs was made using the GO and KEGG databases. Comparison of these translated proteins with those of four shallow-water amphipods revealed 10,731 gene families, of which 5659 were single-copy orthologs. Base substitution analysis on these single-copy orthologs showed that 62 genes are positively selected in H. gigas, including genes related to beta-alanine biosynthesis, energy metabolism and genetic information processing. For multiple-copy orthologous genes, gene family expansion analysis revealed that cold-inducible proteins (i.e., transcription factors II A and transcription elongation factor 1) as well as zinc finger domains are expanded in H. gigas. Overall, our results indicate that genetic adaptation to the hadal environment by H. gigas may be mediated by both gene family expansion and amino acid substitutions of specific proteins.
SubtypeArticle
KeywordChallenger Deep Deep Sea Hydrostatic Pressure Mariana Trench Positive Selection
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1111/mec.14149
URL查看原文
Indexed BySCI
Language英语
WOS KeywordHELICASE-II GU ; HYDROSTATIC-PRESSURE ; POSITIVE SELECTION ; RNA HELICASE ; MAXIMUM-LIKELIHOOD ; HIGH-THROUGHPUT ; MARIANA TRENCH ; PROTEIN ; GENOME ; ANNOTATION
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS IDWOS:000404618000013
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/3962
Collection深海科学研究部_深海生物学研究室_深海无脊椎动物蛋白质组学及分子生物学研究组
Corresponding AuthorQian, Pei-Yuan; Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China)
Affiliation1.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China
2.Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA
3.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China
4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China
Recommended Citation
GB/T 7714
Lan, Yi,Sun, Jin,Tian, Renmao,et al. Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas[J]. MOLECULAR ECOLOGY,2017,26(14):3732-3743.
APA Lan, Yi.,Sun, Jin.,Tian, Renmao.,Bartlett, Douglas H..,Li, Runsheng.,...&Qian, Pei-Yuan.(2017).Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas.MOLECULAR ECOLOGY,26(14),3732-3743.
MLA Lan, Yi,et al."Molecular adaptation in the world's deepest-living animal: Insights from transcriptome sequencing of the hadal amphipod Hirondellea gigas".MOLECULAR ECOLOGY 26.14(2017):3732-3743.
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