A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila
Liu, Helu; Wang, Hui; Cai, Shanya; Zhang, Haibin; Zhang HB(张海滨); Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
2017-08-01
发表期刊MARINE BIOTECHNOLOGY
卷号19期号:4页码:345-350
产权排序第1完成单位 ; 第1完成单位
摘要One paradox of the trophic biochemistry of the deep sea giant tubeworm Riftia pachyptila, endemic to hydrothermal vent sites and nourished by polyunsaturated fatty acid (PUFA) deficiency chemolitoautotrophic sulfide-oxidizing bacteria, is the source of their PUFAs. Biosynthesis of PUFA starts with two precursors C18:2n-6 and C18:3n-3, which cannot be biosynthesized by most animals due to lack of omega 6- and omega 3-desaturase; thus, C18:2n-6 and C18:3n-3 are generally essential fatty acids for animals. Here, we characterized a gene derived from the R. pachyptila located by hydrothermal vent, which encoded a novel omega 3-desaturase (Rp3Fad). The gene was identified by searching the R. pachyptila transcriptome database using known omega 3-desaturases, and its predicted protein showed 37-45% identical to omega 3-desaturases of fungus and microalgae, and only 31% identitical to nematode Caenorhabditis elegans omega 3-desaturase. Expression in yeast Saccharomyces cerevisiae showed that the Rp3Fad could desaturate C18:2n-6 and C18:3n-6 into C18:3n-3 and C18:4n-3, respectively, displaying a Delta 15 activity similar to plant omega 3-desaturase, but it showed no activity towards C20 n-6 PUFA substrates, differing from the well-characterized C. elegans omega 3-desaturases Delta 5, Delta 6, Delta 8, and Delta 12 activity were also tested, resulting in no corresponding production. The function of omega 3-desaturase identified in R. pachyptila could produce C18:3n - 3 used in synthesis of n - 3 series PUFAs, suggesting an adaption to PUFA deficiency environment in deep sea hydrothermal vent.
文章类型Article
关键词Giant Tubeworm Riftia Pachyptila Pufa Biosynthesis Fatty Acid Deep Sea Hydrothermal Vent
学科领域分子生物学
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10126-017-9753-9
URL查看原文 ; 查看原文 ; 查看原文
收录类别SCI
语种英语
关键词[WOS]POLYUNSATURATED FATTY-ACIDS ; MULTI-HEMOGLOBIN SYSTEM ; OMEGA-3-FATTY-ACID DESATURASE ; CAENORHABDITIS-ELEGANS ; LIPID BIOMARKERS ; BIOSYNTHESIS ; BACTERIA ; MECHANISMS ; SYMBIONTS ; BIVALVES
WOS类目Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号WOS:000406962700003
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.idsse.ac.cn/handle/183446/2600
专题深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者Zhang, Haibin; Zhang HB(张海滨); Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
作者单位Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
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Liu, Helu,Wang, Hui,Cai, Shanya,et al. A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila[J]. MARINE BIOTECHNOLOGY,2017,19(4):345-350.
APA Liu, Helu,Wang, Hui,Cai, Shanya,Zhang, Haibin,张海滨,&Zhang, Haibin.(2017).A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila.MARINE BIOTECHNOLOGY,19(4),345-350.
MLA Liu, Helu,et al."A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila".MARINE BIOTECHNOLOGY 19.4(2017):345-350.
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