| A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila | |
Liu, Helu; Wang, Hui; Cai, Shanya; Zhang, Haibin ; Zhang HB(张海滨); Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
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| 2017-08-01 | |
| 发表期刊 | MARINE BIOTECHNOLOGY
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| 卷号 | 19期号:4页码:345-350 |
| 产权排序 | 第1完成单位 ; 第1完成单位 |
| 摘要 | One paradox of the trophic biochemistry of the deep sea giant tubeworm Riftia pachyptila, endemic to hydrothermal vent sites and nourished by polyunsaturated fatty acid (PUFA) deficiency chemolitoautotrophic sulfide-oxidizing bacteria, is the source of their PUFAs. Biosynthesis of PUFA starts with two precursors C18:2n-6 and C18:3n-3, which cannot be biosynthesized by most animals due to lack of omega 6- and omega 3-desaturase; thus, C18:2n-6 and C18:3n-3 are generally essential fatty acids for animals. Here, we characterized a gene derived from the R. pachyptila located by hydrothermal vent, which encoded a novel omega 3-desaturase (Rp3Fad). The gene was identified by searching the R. pachyptila transcriptome database using known omega 3-desaturases, and its predicted protein showed 37-45% identical to omega 3-desaturases of fungus and microalgae, and only 31% identitical to nematode Caenorhabditis elegans omega 3-desaturase. Expression in yeast Saccharomyces cerevisiae showed that the Rp3Fad could desaturate C18:2n-6 and C18:3n-6 into C18:3n-3 and C18:4n-3, respectively, displaying a Delta 15 activity similar to plant omega 3-desaturase, but it showed no activity towards C20 n-6 PUFA substrates, differing from the well-characterized C. elegans omega 3-desaturases Delta 5, Delta 6, Delta 8, and Delta 12 activity were also tested, resulting in no corresponding production. The function of omega 3-desaturase identified in R. pachyptila could produce C18:3n - 3 used in synthesis of n - 3 series PUFAs, suggesting an adaption to PUFA deficiency environment in deep sea hydrothermal vent. |
| 文章类型 | Article |
| 关键词 | Giant Tubeworm Riftia Pachyptila Pufa Biosynthesis Fatty Acid Deep Sea Hydrothermal Vent |
| 学科领域 | 分子生物学 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s10126-017-9753-9 |
| URL | 查看原文 ; 查看原文 ; 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | POLYUNSATURATED FATTY-ACIDS ; MULTI-HEMOGLOBIN SYSTEM ; OMEGA-3-FATTY-ACID DESATURASE ; CAENORHABDITIS-ELEGANS ; LIPID BIOMARKERS ; BIOSYNTHESIS ; BACTERIA ; MECHANISMS ; SYMBIONTS ; BIVALVES |
| WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000406962700003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.idsse.ac.cn/handle/183446/2600 |
| 专题 | 深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组 |
| 通讯作者 | Zhang, Haibin; Zhang HB(张海滨); Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China) |
| 作者单位 | Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Helu,Wang, Hui,Cai, Shanya,et al. A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila[J]. MARINE BIOTECHNOLOGY,2017,19(4):345-350. |
| APA | Liu, Helu,Wang, Hui,Cai, Shanya,Zhang, Haibin,张海滨,&Zhang, Haibin.(2017).A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila.MARINE BIOTECHNOLOGY,19(4),345-350. |
| MLA | Liu, Helu,et al."A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila".MARINE BIOTECHNOLOGY 19.4(2017):345-350. |
| 条目包含的文件 | ||||||
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| liuhelu.pdf（989KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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