A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila

doi:10.1007/s10126-017-9753-9

IDSSE OpenIR > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila
	Liu, Helu; Wang, Hui; Cai, Shanya; Zhang, Haibin; Zhang HB(张海滨); Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
	2017-08-01
发表期刊	MARINE BIOTECHNOLOGY
卷号	19 期号:4 页码:345-350
产权排序	第1完成单位 ; 第1完成单位
摘要	One paradox of the trophic biochemistry of the deep sea giant tubeworm Riftia pachyptila, endemic to hydrothermal vent sites and nourished by polyunsaturated fatty acid (PUFA) deficiency chemolitoautotrophic sulfide-oxidizing bacteria, is the source of their PUFAs. Biosynthesis of PUFA starts with two precursors C18:2n-6 and C18:3n-3, which cannot be biosynthesized by most animals due to lack of omega 6- and omega 3-desaturase; thus, C18:2n-6 and C18:3n-3 are generally essential fatty acids for animals. Here, we characterized a gene derived from the R. pachyptila located by hydrothermal vent, which encoded a novel omega 3-desaturase (Rp3Fad). The gene was identified by searching the R. pachyptila transcriptome database using known omega 3-desaturases, and its predicted protein showed 37-45% identical to omega 3-desaturases of fungus and microalgae, and only 31% identitical to nematode Caenorhabditis elegans omega 3-desaturase. Expression in yeast Saccharomyces cerevisiae showed that the Rp3Fad could desaturate C18:2n-6 and C18:3n-6 into C18:3n-3 and C18:4n-3, respectively, displaying a Delta 15 activity similar to plant omega 3-desaturase, but it showed no activity towards C20 n-6 PUFA substrates, differing from the well-characterized C. elegans omega 3-desaturases Delta 5, Delta 6, Delta 8, and Delta 12 activity were also tested, resulting in no corresponding production. The function of omega 3-desaturase identified in R. pachyptila could produce C18:3n - 3 used in synthesis of n - 3 series PUFAs, suggesting an adaption to PUFA deficiency environment in deep sea hydrothermal vent.
文章类型	Article
关键词	Giant Tubeworm Riftia Pachyptila Pufa Biosynthesis Fatty Acid Deep Sea Hydrothermal Vent
学科领域	分子生物学
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s10126-017-9753-9
URL	查看原文 ; 查看原文 ; 查看原文
收录类别	SCI
语种	英语
关键词[WOS]	POLYUNSATURATED FATTY-ACIDS ; MULTI-HEMOGLOBIN SYSTEM ; OMEGA-3-FATTY-ACID DESATURASE ; CAENORHABDITIS-ELEGANS ; LIPID BIOMARKERS ; BIOSYNTHESIS ; BACTERIA ; MECHANISMS ; SYMBIONTS ; BIVALVES
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000406962700003
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.idsse.ac.cn/handle/183446/2600
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin; Zhang HB(张海滨); Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
作者单位	Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
推荐引用方式 GB/T 7714	Liu, Helu,Wang, Hui,Cai, Shanya,et al. A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila[J]. MARINE BIOTECHNOLOGY,2017,19(4):345-350.
APA	Liu, Helu,Wang, Hui,Cai, Shanya,Zhang, Haibin,张海滨,&Zhang, Haibin.(2017).A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila.MARINE BIOTECHNOLOGY,19(4),345-350.
MLA	Liu, Helu,et al."A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila".MARINE BIOTECHNOLOGY 19.4(2017):345-350.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
liuhelu.pdf（989KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	请求全文