A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila
Liu, Helu; Wang, Hui; Cai, Shanya; Zhang, Haibin; Zhang HB(张海滨); Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
2017-08-01
Source PublicationMARINE BIOTECHNOLOGY
Volume19Issue:4Pages:345-350
Contribution Rank第1完成单位 ; 第1完成单位
AbstractOne paradox of the trophic biochemistry of the deep sea giant tubeworm Riftia pachyptila, endemic to hydrothermal vent sites and nourished by polyunsaturated fatty acid (PUFA) deficiency chemolitoautotrophic sulfide-oxidizing bacteria, is the source of their PUFAs. Biosynthesis of PUFA starts with two precursors C18:2n-6 and C18:3n-3, which cannot be biosynthesized by most animals due to lack of omega 6- and omega 3-desaturase; thus, C18:2n-6 and C18:3n-3 are generally essential fatty acids for animals. Here, we characterized a gene derived from the R. pachyptila located by hydrothermal vent, which encoded a novel omega 3-desaturase (Rp3Fad). The gene was identified by searching the R. pachyptila transcriptome database using known omega 3-desaturases, and its predicted protein showed 37-45% identical to omega 3-desaturases of fungus and microalgae, and only 31% identitical to nematode Caenorhabditis elegans omega 3-desaturase. Expression in yeast Saccharomyces cerevisiae showed that the Rp3Fad could desaturate C18:2n-6 and C18:3n-6 into C18:3n-3 and C18:4n-3, respectively, displaying a Delta 15 activity similar to plant omega 3-desaturase, but it showed no activity towards C20 n-6 PUFA substrates, differing from the well-characterized C. elegans omega 3-desaturases Delta 5, Delta 6, Delta 8, and Delta 12 activity were also tested, resulting in no corresponding production. The function of omega 3-desaturase identified in R. pachyptila could produce C18:3n - 3 used in synthesis of n - 3 series PUFAs, suggesting an adaption to PUFA deficiency environment in deep sea hydrothermal vent.
SubtypeArticle
KeywordGiant Tubeworm Riftia Pachyptila Pufa Biosynthesis Fatty Acid Deep Sea Hydrothermal Vent
Subject Area分子生物学
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10126-017-9753-9
URL查看原文 ; 查看原文 ; 查看原文
Indexed BySCI
Language英语
WOS KeywordPOLYUNSATURATED FATTY-ACIDS ; MULTI-HEMOGLOBIN SYSTEM ; OMEGA-3-FATTY-ACID DESATURASE ; CAENORHABDITIS-ELEGANS ; LIPID BIOMARKERS ; BIOSYNTHESIS ; BACTERIA ; MECHANISMS ; SYMBIONTS ; BIVALVES
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000406962700003
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/2600
Collection深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
Corresponding AuthorZhang, Haibin; Zhang HB(张海滨); Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China)
AffiliationChinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Corresponding Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
Liu, Helu,Wang, Hui,Cai, Shanya,et al. A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila[J]. MARINE BIOTECHNOLOGY,2017,19(4):345-350.
APA Liu, Helu,Wang, Hui,Cai, Shanya,Zhang, Haibin,张海滨,&Zhang, Haibin.(2017).A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila.MARINE BIOTECHNOLOGY,19(4),345-350.
MLA Liu, Helu,et al."A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila".MARINE BIOTECHNOLOGY 19.4(2017):345-350.
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