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Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring
Chen, Shun; Peng, Xiaotong; Xu, Hengchao; Ta, Kaiwen
Source PublicationBIOGEOSCIENCES

The oxidation of ammonia by microbes has been shown to occur in diverse natural environments. However, the link of in situ nitrification activity to taxonomic identities of ammonia oxidizers in high-temperature environments remains poorly understood. Here, we studied in situ ammonia oxidation rates and the diversity of ammonia-oxidizing Archaea (AOA) in surface and bottom sediments at 77 degrees C in the Gongxiaoshe hot spring, Tengchong, Yunnan, China. The in situ ammonia oxidation rates measured by the N-15-NO3- pool dilution technique in the surface and bottom sediments were 4.80 and 5.30 nmol N g(-1) h(-1), respectively. Real-time quantitative polymerase chain reaction (qPCR) indicated that the archaeal 16S rRNA genes and amoA genes were present in the range of 0.128 to 1.96 x 10(8) and 2.75 to 9.80 x 10(5) gene copies g(-1) sediment, respectively, while bacterial amoA was not detected. Phylogenetic analysis of 16S rRNA genes showed high sequence similarity to thermophilic Candidatus Nitrosocaldus yellowstonii, which represented the most abundant operational taxonomic units (OTU) in both surface and bottom sediments. The archaeal predominance was further supported by fluorescence in situ hybridization (FISH) visualization. The cell-specific rate of ammonia oxidation was estimated to range from 0.410 to 0.790 fmolNarchaeal cell(-1) h(-1), higher than those in the two US Great Basin hot springs. These results suggest the importance of archaeal rather than bacterial ammonia oxidation in driving the nitrogen cycle in terrestrial geothermal environments.

WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
WOS Keyword16s Ribosomal-rna ; Nitrogen-cycle ; Amoa Genes ; Agricultural Soil ; Important Players ; Yunnan Province ; Oxidation ; Bacteria ; Crenarchaeota ; Abundance
WOS SubjectEcology ; Geosciences, Multidisciplinary
WOS IDWOS:000377276000005
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
AffiliationChinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
First Author AffilicationInstitute of Deep-sea Science and Engineering,CAS
Recommended Citation
GB/T 7714
Chen, Shun,Peng, Xiaotong,Xu, Hengchao,et al. Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring[J]. BIOGEOSCIENCES,2016,13(7):2051-2060.
APA Chen, Shun,Peng, Xiaotong,Xu, Hengchao,&Ta, Kaiwen.(2016).Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring.BIOGEOSCIENCES,13(7),2051-2060.
MLA Chen, Shun,et al."Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring".BIOGEOSCIENCES 13.7(2016):2051-2060.
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