Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200
Zhang, Sheng-Da1,3,4; Santini, Claire-Lise2,3,4; Zhang, Wei-Jia1,3,4; Barbe, Valerie5; Mangenot, Sophie5; Guyomar, Charlotte2,3,4; Garel, Marc6; Chen, Hai-Tao1,3,4; Li, Xue-Gong1,3,4; Yin, Qun-Jian1,3,4; Zhao, Yuan7; Armengaud, Jean8; Gaillard, Jean-Charles8; Martini, Severine6; Pradel, Nathalie6; Vidaud, Claude8; Alberto, Francois2,3,4; Medigue, Claudine9,10,11; Tamburini, Christian6; Wu, Long-Fei2,3,4
2016-05-01
Source PublicationEXTREMOPHILES
Volume20Issue:3Pages:301-310
AbstractBacteria of the genus Photobacterium thrive worldwide in oceans and show substantial eco-physiological diversity including free-living, symbiotic and piezophilic life styles. Genomic characteristics underlying this variability across species are poorly understood. Here we carried out genomic and physiological analysis of Photobacterium phosphoreum strain ANT-2200, the first deep-sea luminous bacterium of which the genome has been sequenced. Using optical mapping we updated the genomic data and reassembled it into two chromosomes and a large plasmid. Genomic analysis revealed a versatile energy metabolic potential and physiological analysis confirmed its growth capacity by deriving energy from fermentation of glucose or maltose, by respiration with formate as electron donor and trimethlyamine N-oxide (TMAO), nitrate or fumarate as electron acceptors, or by chemo-organo-heterotrophic growth in rich media. Despite that it was isolated at a site with saturated dissolved oxygen, the ANT-2200 strain possesses four gene clusters coding for typical anaerobic enzymes, the TMAO reductases. Elevated hydrostatic pressure enhances the TMAO reductase activity, mainly due to the increase of isoenzyme TorA1. The high copy number of the TMAO reductase isoenzymes and pressure-enhanced activity might imply a strategy developed by bacteria to adapt to deep-sea habitats where the instant TMAO availability may increase with depth.
SubtypeArticle
KeywordDeep-sea Adaptation Bioluminescence Tmao Reductase Hydrostatic Pressure Anaerobic Respiration
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00792-016-0822-1
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Indexed BySCI
Funding OrganizationThis work was supported by Grants SIDSSE-201307, SIDSSE-QN-201405, SIDSSE-QN-201406 and SIDSSE-QN-201408 from Sanya Institute of Deep-Sea Sciences and Engineering, the Strategic Priority Research Program grant XDB06010203 and International Partnership for Innovative Team Program (20140491526) from the Chinese Academy of Sciences, the NSFC 41506147 from National Natural Science Foundation of China, a grant for LIA-BioMNSL from Centre National de la Recherche Scientifique, the grant DY125-15-R-03 from China Ocean Mineral Resources R & D Association (COMRA) Special Foundation, the Grant NSFC 41306161 from the National Science Foundation of China and a grant from Mt. Tai Scholar Construction Engineering Special Foundation of Shandong Province. We acknowledge France Genomique for the support for this sequencing project.
Language英语
WOS KeywordESCHERICHIA-COLI ; RESPONSE REGULATOR ; OPERON EXPRESSION ; MARINE BACTERIUM ; PROFUNDUM SS9 ; STRAIN MO-1 ; PRESSURE ; TORR ; ADAPTATION ; SEQUENCE
WOS SubjectBiochemistry & Molecular Biology ; Microbiology
WOS IDWOS:000374832100007
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/1542
Collection深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
研究生部
Affiliation1.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Dept Deep Sea Sci, Deep Sea Microbial Cell Biol, Sanya, Peoples R China
2.Aix Marseille Univ, CNRS, IMM, LCB UMR 7257, 31 Chem Joseph Aiguier, F-13402 Marseille 20, France
3.LCB CNRS, France China Biomineralizat & Nanostruct Lab, LIA BioMNSL, Marseille, France
4.Chinese Acad Sci, SIDSSE, Sanya, Peoples R China
5.CEA, DSV IG Genoscope LF, Evry, France
6.Univ Sud Toulon Var, Aix Marseille Univ, CNRS INSU, IRD,MIO,UM110, F-13288 Marseille, France
7.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
8.CEA, DSV, IBEB, SBTN, Bagnols Sur Ceze, France
9.CEA, DSV IG Genoscope, Lab Analyse Bioinformat Genom & Metab, Evry, France
10.CNRS, UMR 8030, Evry, France
11.Univ Evry Val Esssone, Evry, France
Recommended Citation
GB/T 7714
Zhang, Sheng-Da,Santini, Claire-Lise,Zhang, Wei-Jia,et al. Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200[J]. EXTREMOPHILES,2016,20(3):301-310.
APA Zhang, Sheng-Da.,Santini, Claire-Lise.,Zhang, Wei-Jia.,Barbe, Valerie.,Mangenot, Sophie.,...&Wu, Long-Fei.(2016).Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200.EXTREMOPHILES,20(3),301-310.
MLA Zhang, Sheng-Da,et al."Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200".EXTREMOPHILES 20.3(2016):301-310.
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