siRNA transfection in larvae of the barnacle Amphibalanus amphitrite
Zhang, Gen1,2; He, Li-Sheng2,3; Wong, Yue Him2; Yu, Li1,2; Qian, Pei-Yuan1,2
2015-08-01
Source PublicationJOURNAL OF EXPERIMENTAL BIOLOGY
Volume218Issue:16Pages:2505-2509
AbstractRNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in 'double transfections', in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. A relatively longer transfection time and more larval cells directly exposed to siRNA might explain the higher efficiency of double transfection experiments.
SubtypeArticle
KeywordTransfection P38 Mapk Rna Interference Amphibalanus Amphitrite Larval Settlement
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1242/jeb.120113
Indexed BySCI
Language英语
WOS KeywordDISTAL-LESS ; RNA INTERFERENCE ; GENE ; EXPRESSION ; MODULATION ; KNOCKDOWN ; SYSTEM ; DSRNA ; PCR
WOS SubjectBiology
WOS IDWOS:000359937300007
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.idsse.ac.cn/handle/183446/1213
Collection深海科学研究部_深海生物学研究室_深海无脊椎动物蛋白质组学及分子生物学研究组
Affiliation1.Hong Kong Univ Sci & Technol, Sch Sci, Environm Sci Programs, Kowloon, Hong Kong, Peoples R China
2.Hong Kong Univ Sci & Technol, Sch Sci, Div Life Sci, KAUST Global Collaborat Res Program, Kowloon, Hong Kong, Peoples R China
3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya City 572000, Hainan Province, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Gen,He, Li-Sheng,Wong, Yue Him,et al. siRNA transfection in larvae of the barnacle Amphibalanus amphitrite[J]. JOURNAL OF EXPERIMENTAL BIOLOGY,2015,218(16):2505-2509.
APA Zhang, Gen,He, Li-Sheng,Wong, Yue Him,Yu, Li,&Qian, Pei-Yuan.(2015).siRNA transfection in larvae of the barnacle Amphibalanus amphitrite.JOURNAL OF EXPERIMENTAL BIOLOGY,218(16),2505-2509.
MLA Zhang, Gen,et al."siRNA transfection in larvae of the barnacle Amphibalanus amphitrite".JOURNAL OF EXPERIMENTAL BIOLOGY 218.16(2015):2505-2509.
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