| Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii | |
Li, Xuegong1,2 ; Fu, Ling1; Li, Zhen1; Ma, Xiaopan1; Xiao, Xiang1,3,4; Xu, Jun1,3,4
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| 2015 | |
| Source Publication | EXTREMOPHILES
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| Volume | 19Issue:1Pages:59-67 |
| Abstract | The hyperthermophile Pyrococcus yayanosii CH1 is the only high-pressure-requiring microorganism obtained thus far within the archaea domain or among all non-psychrophiles in any domain. In this study, we developed a genetic manipulation system for P. yayanosii after first isolating a facultatively piezophilic derivative strain, designated P. yayanosii A1. The 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene was overexpressed in strain P. yayanosii A1 and was demonstrated to confer host cell resistance against simvastatin. Furthermore, using simvastatin as a selection marker, the endogenous pyrF of P. yayanosii A1 was disrupted through homologous recombination, thus generating the additional host strain P. yayanosii A2 (Delta pyrF). A markerless gene disruption vector was constructed by incorporating a pyrF-sim (R) cassette that enables the combined use of simvastatin resistance for positive selection and 5-FOA for counter selection. The utility of this versatile disruption system was demonstrated by deleting the carbon-nitrogen hydrolase of P. yayanosii strain A1. These results demonstrate that a variety of genetic tools are now in place to study unknown gene function and the molecular mechanisms of piezophilic adaptation in P. yayanosii. |
| Subtype | Article |
| Keyword | Piezophile Genetic Tool Pyrococcus Yayanosii Hyperthermophilic Archaeon |
| Subject Area | 海洋生物学 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s00792-014-0705-2 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | Piezophile ; Genetic tool ; Pyrococcus yayanosii ; Hyperthermophilic archaeon |
| WOS Subject | Biochemistry & Molecular Biology ; Microbiology |
| WOS ID | WOS:000346657500006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.idsse.ac.cn/handle/183446/1204 |
| Collection | 深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组 |
| Affiliation | 1.Shanghai Jiao Tong Univ, Inst Oceanol, Shanghai 200240, Peoples R China 2.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Dept Deep Sea Sci, Sanya 572000, Peoples R China 3.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Xuegong,Fu, Ling,Li, Zhen,et al. Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii[J]. EXTREMOPHILES,2015,19(1):59-67. |
| APA | Li, Xuegong,Fu, Ling,Li, Zhen,Ma, Xiaopan,Xiao, Xiang,&Xu, Jun.(2015).Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii.EXTREMOPHILES,19(1),59-67. |
| MLA | Li, Xuegong,et al."Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii".EXTREMOPHILES 19.1(2015):59-67. |
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