Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii
Li, Xuegong1,2; Fu, Ling1; Li, Zhen1; Ma, Xiaopan1; Xiao, Xiang1,3,4; Xu, Jun1,3,4
2015
Source PublicationEXTREMOPHILES
Volume19Issue:1Pages:59-67
AbstractThe hyperthermophile Pyrococcus yayanosii CH1 is the only high-pressure-requiring microorganism obtained thus far within the archaea domain or among all non-psychrophiles in any domain. In this study, we developed a genetic manipulation system for P. yayanosii after first isolating a facultatively piezophilic derivative strain, designated P. yayanosii A1. The 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene was overexpressed in strain P. yayanosii A1 and was demonstrated to confer host cell resistance against simvastatin. Furthermore, using simvastatin as a selection marker, the endogenous pyrF of P. yayanosii A1 was disrupted through homologous recombination, thus generating the additional host strain P. yayanosii A2 (Delta pyrF). A markerless gene disruption vector was constructed by incorporating a pyrF-sim (R) cassette that enables the combined use of simvastatin resistance for positive selection and 5-FOA for counter selection. The utility of this versatile disruption system was demonstrated by deleting the carbon-nitrogen hydrolase of P. yayanosii strain A1. These results demonstrate that a variety of genetic tools are now in place to study unknown gene function and the molecular mechanisms of piezophilic adaptation in P. yayanosii.
SubtypeArticle
KeywordPiezophile Genetic Tool Pyrococcus Yayanosii Hyperthermophilic Archaeon
Subject Area海洋生物学
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00792-014-0705-2
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPiezophile ; Genetic tool ; Pyrococcus yayanosii ; Hyperthermophilic archaeon
WOS SubjectBiochemistry & Molecular Biology ; Microbiology
WOS IDWOS:000346657500006
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.idsse.ac.cn/handle/183446/1204
Collection深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
Affiliation1.Shanghai Jiao Tong Univ, Inst Oceanol, Shanghai 200240, Peoples R China
2.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Dept Deep Sea Sci, Sanya 572000, Peoples R China
3.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
Li, Xuegong,Fu, Ling,Li, Zhen,et al. Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii[J]. EXTREMOPHILES,2015,19(1):59-67.
APA Li, Xuegong,Fu, Ling,Li, Zhen,Ma, Xiaopan,Xiao, Xiang,&Xu, Jun.(2015).Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii.EXTREMOPHILES,19(1),59-67.
MLA Li, Xuegong,et al."Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii".EXTREMOPHILES 19.1(2015):59-67.
Files in This Item: Download All
File Name/Size DocType Version Access License
2015-Genetic tools f(835KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Xuegong]'s Articles
[Fu, Ling]'s Articles
[Li, Zhen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Xuegong]'s Articles
[Fu, Ling]'s Articles
[Li, Zhen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Xuegong]'s Articles
[Fu, Ling]'s Articles
[Li, Zhen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 2015-Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.